摘要
目的:探究罗哌卡因对骨肉瘤细胞增殖、侵袭及雷帕霉素靶蛋白(mTOR)/缺氧诱导因子1α(HIF-1α)信号通路的影响。方法:将MG63细胞分为对照组、罗哌卡因低浓度组、罗哌卡因中浓度组、罗哌卡因高浓度组。采用平板克隆法测定MG63细胞平板克隆率,采用免疫组化法测定MG63细胞Ki-67表达,采用Transwell法测定MG63细胞侵袭、迁移能力,采用Western blot法测定MG63细胞基质金属蛋白酶2(MMP-2)、MMP-9、mTOR、HIF-1α蛋白表达。结果:与对照组比较,罗哌卡因低、中、高浓度组MG63细胞克隆形成率、Ki-67阳性率、侵袭细胞数、迁移细胞数以及MMP-2、MMP-9、mTOR、HIF-1α蛋白表达降低(均P<0.05)。与罗哌卡因低浓度组比较,罗哌卡因中、高浓度组MG63细胞克隆形成率、Ki-67阳性率、侵袭细胞数、迁移细胞数以及MMP-2、MMP-9、mTOR、HIF-1α蛋白表达降低(均P<0.05)。与罗哌卡因中浓度组比较,罗哌卡因高浓度组MG63细胞克隆形成率、Ki-67阳性率、侵袭细胞数、迁移细胞数以及MMP-2、MMP-9、mTOR、HIF-1α蛋白表达降低(均P<0.05)。结论:罗哌卡因可抑制MG63细胞增殖、侵袭、迁移,并可抑制mTOR/HIF-1α通路的激活。
Objective:To investigate the effects of ropivacaine on the proliferation,invasion and mammalian target of rapamycin(mTOR)/hypoxia-inducible factor 1α(HIF-1α)signaling pathway of osteosarcoma cells.Methods:MG63 cells were grouped into control group,low-concentration ropivacaine group,medium-concentration ropivacaine group,and high-concentration ropivacaine group.Plate cloning method was applied to determine the plate cloning rate of MG63 cells.Immunohistochemistry was used to determine the expression of Ki-67 in MG63 cells.Transwell method was applied to determine the invasion and migration abilities of MG63 cells.Western blot was applied to determine the protein expressions of Matrix metalloproteinase 2(MMP-2),MMP-9,mTOR and HIF-1αin MG63 cells.Results:Compared with the control group,the colony formation rate,the positive rate of Ki-67,the number of invasive cells,the number of migrating cells,the protein expressions of MMP-2,MMP-9,mTOR and HIF-1αdecreased in the low-,medium-and high-concentration ropivacaine group(all P<0.05).Compared with the low-concentration ropivacaine group,the colony formation rate,the positive rate of Ki-67,the number of invasive cells,the number of migrating cells,the protein expressions of MMP-2,MMP-9,mTOR and HIF-1αdecreased in the medium-and high-concentration ropivacaine group(all P<0.05).Compared with the medium-concentration ropivacaine group,the colony formation rate,the positive rate of Ki-67,the number of invasive cells,the number of migrating cells,the protein expressions of MMP-2,MMP-9,mTOR and HIF-1αdecreased in the high-concentration ropivacaine group(all P<0.05).Conclusion:Ropivacaine can inhibit the proliferation,invasion and migration of MG63 cells,and can inhibit the activation of mTOR/HIF-1αpathway.
作者
张素冰
高辉
赵滨滨
张丹琦
ZHANG Subing;GAO Hui;ZHAO Binbin;ZHANG Danqi(Department of Anesthesiology and Surgery,First Affiliated Hospital,Heilongjiang University of Chinese Medicine,Harbin 150040,China)
出处
《陕西医学杂志》
CAS
2023年第6期666-670,共5页
Shaanxi Medical Journal
基金
黑龙江省中医药科研项目(ZHY2020-129)。
关键词
骨肉瘤
罗哌卡因
增殖
侵袭
雷帕霉素靶蛋白
缺氧诱导因子1Α
Osteosarcoma
Ropivacaine
Proliferation
Invasion
Mammalian target of rapamycin
Hypoxia inducible factor 1α
