摘要
目的:通过灌喂硫酸亚铁溶液构建氧化应激小鼠模型,研究不同灌喂剂量花青素对氧化应激小鼠生长、脂代谢和肝脏抗氧化能力的影响。方法:选择21日龄平均体质量15 g的雄性小鼠40只,按体质量相近原则分为C组(对照组,0.5 mL生理盐水)、I组(生理盐水+FeSO 4混合溶液)、II组(生理盐水+FeSO_(4)溶液+100 mg/kg花青素的混合溶液)和III组(生理盐水+FeSO 4溶液+300 mg/kg花青素的混合溶液),试验小鼠连续灌喂2周,测定小鼠生长、器官指数、脂代谢、肝脏功能、肝脏氧化标记物和抗氧化酶等指标。结果:与C和II组相比,III组小鼠的增重显著降低(P<0.05);与I组相比,C和III组小鼠肝脏指数显著降低(P<0.05)、II和III组小鼠的附睾脂肪指数极显著降低(P<0.01);III组小鼠血清甘油三酯水平较其他组显著降低(P<0.05),与C组相比,II和III组小鼠血清高密度脂蛋白水平显著降低(P<0.05);I组小鼠血清谷丙转氨酶和谷草转氨酶活性较其他组显著升高(P<0.05);与C组相比,I~III组小鼠肝脏组织丙二醛水平极显著升高(P<0.01),与III组相比,I组小鼠肝脏组织超氧化物歧化酶活性和总抗氧化能力显著升高(P<0.05),I组小鼠肝脏组织过氧化氢酶活性较其他组显著降低(P<0.05)。III组小鼠肝脏组织谷胱甘肽过氧化物酶活性较其他组显著降低(P<0.05)。结论:灌喂硫酸亚铁溶液增加了小鼠肝脏指数,影响小鼠脂代谢,提高了肝脏抗氧化能力;灌喂花青素溶液(300 mg/kg)降低小鼠体增重,提高小鼠脾脏指数,增强氧化应激小鼠机体细胞免疫机能,提高了胆固醇逆向转运至肝脏分解的能力;花青素对小鼠机体血脂代谢和缓解硫酸亚铁溶液引起的肝脏功能损伤存在积极作用。
Objective:An oxidative stress mouse model was constructed by intragastric administration exposure to solutions of ferrous sulfate(FeSO_(4)),and the effect of intragastric administration of varying doses of anthocyanin on growth,lipid metabolism,and hepatic antioxidant capacity of oxidative stress mouse model were investigated.Methods:Forty healthy male mice(15 g,21 days old)were selected and randomly divided into four groups,the Group C(control group,0.5 mL saline),the Group I(saline+FeSO_(4)),the Group II(saline+FeSO_(4)+100 mg/kg anthocyanin),and the Group III(saline+FeSO_(4)+300 mg/kg anthocyanin).The intragastric administration in 2 consecutive weeks.The growth,organ index,lipid metabolism,liver function,and hepatic oxidative stress marker as antioxidant enzymes were further analyzed.Results:Compared with Group C and II,the weight gain was significantly decreased in Group III mice(P<0.05).Compared with Group I,the liver index in Group C and III mice(P<0.05)as well as the epididymal adipose index in Group II and III mice(P<0.01)were significantly decreased.The triglycerides in Group III mice were significantly decreased than other groups(P<0.05).Compared with Group C,the high-density lipoprotein in Group II and III mice was significantly decreased(P<0.05).The alanine aminotransferase and aspartate aminotransferase in Group I mice were significantly increased than other groups(P<0.05).Compared with Group C,the hepatic malondialdehyde in Groups I—III mice were significantly increased(P<0.01).Compared with Group III,the hepatic superoxide dismutase and total anti-oxidant capacity in Group I mice were significantly increased(P<0.05).The hepatic catalase in Group I mice was significantly decreased(P<0.05).The hepatic glutathione peroxidase in Group III mice was significantly decreased(P<0.05).Conclusion:Intragastric administration exposure to solutions of ferrous sulfate increased the liver index in model mice,which also affect lipid metabolism and improve the antioxidant capacity of the liver.intragastric administration of anthocyanin(300 mg/kg)decreased the weight gain of model mice,enhancing the immune function of mice by increasing the splenic index,which also increased the reverse transport capacity of cholesterol back to the liver.Finally,anthocyanin has a positive effect on the regulation of lipid metabolism and alleviates the hepatic functional disorders caused by ferrous sulfate solutions.
作者
张峰
林志勇
刘晓丹
闻爱友
张子婷
刘纪元
王晓楠
杜民
靳二辉
ZHANG Feng;LIN Zhiyong;LIU Xiaodan;WEN Aiyou;ZHANG Ziting;LIU Jiyuan;WANG Xiaonan;DU Min;JIN Erhui(College of Animal Science,Anhui Science and Technology University,Fengyang 233100,China;Anhui Province Key Laboratory of Animal Nutritional Regulation and Health,Fengyang 233100,China;Fengyang Xiaogang Minyi Land Shares Cooperatives,Fengyang 233100,China)
出处
《安徽科技学院学报》
2023年第3期6-15,共10页
Journal of Anhui Science and Technology University
基金
国家自然科学基金面上项目(32172816)
安徽省自然科学基金(2108085MC117,2208085MC77)
安徽省高校自然科学研究重点项目(KJ2021A0868)
安徽小岗国家农业科技园区科技计划项目(yq202204)
动物营养调控与健康安徽省重点实验室开放课题(APKLANRH202001)
安徽科技学院人才引进项目(DKYJ202101)
国家级大学生创新创业训练计划项目(202210879075)
生猪绿色高效养殖配套关键技术集成研究与示范(881093)。
关键词
花青素
氧化应激
小鼠模型
脂代谢
肝脏功能
Anthocyanins
Oxidative stress
Mouse model
Lipid metabolism
Liver function
