摘要
针对目前国内测量细胞温度的方法较少,且大多数方法会对细胞造成损伤的问题,研究介绍了一种双发射峰热敏性量子点(QDs)的合成方法,可以应用于细胞温度的无损测量。该方法合成的量子点对于温度的敏感性较强,量子点的荧光强度与温度呈线性关系,可测温度范围为10~50℃,覆盖了大多数活细胞的温度(35~40℃)。并优化了测量荧光发射光谱的方法,增加了实验的准确性,与热电偶相比,相关系数提高至0.993。本文合成的碳量子点对人永生化表皮细胞(HaCaT)进行了温度检测和荧光成像。结果显示,对细胞基本无损伤且温度测量结果准确。所提方法合成的碳量子点具有良好的稳定性、生物相容性,且对细胞基本无毒性,对于开发无损测量细胞的纳米温度计有重要意义。
Aiming at the problem that at present there are few methods for measuring cell temperature in China,and most methods will cause damage to cells,a method for synthesis of thermal-sensitive quantum dots(QDs)with dual emission peaks is introduced,which can be applied to non-destructive measurement of cell temperature.The synthesized quantum dots are highly sensitive to temperature.The fluorescence intensity of quantum dots has a linear relationship with temperature.The measurable temperature range is 10~50℃,covering the temperature of 35~40℃of most living cells.The method of measuring the fluorescence emission spectrum is optimized,and the accuracy of the experiment is increased.Compared with the thermocouple,the correlation coefficient is increased to 0.993.The synthesized carbon quantum dots performs temperature detection and fluorescence imaging on HaCaT cell.The results show that there is basically no damage to the cells and the temperature measurement results are accurate.The synthesized carbon quantum dots have good stability,biocompatibility,and are basically nontoxic to cells,which are of great significance for the development of nanometer thermometers for non-destructive measurement of cells.
作者
宋正勋
卞晨煜
SONG Zhengxun;BIAN Chenyu(College of Electronic Information Engineering,Changchun University of Science and Technology,Changchun 130022,China)
出处
《传感器与微系统》
CSCD
北大核心
2023年第6期25-29,共5页
Transducer and Microsystem Technologies
基金
国家重点研发计划资助项目(2017YFC0803806)。
关键词
碳量子点
水热法
人永生化表皮细胞
热敏性
carbon quantum dots(CQDs)
hydrothermal method
HaCaT cell
thermal-sensitive
