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基于16S rDNA测序分析枳术丸水煎液对慢传输型便秘脾虚证模型小鼠肠道菌群的影响 被引量:1

Effects of Zhizhuwan Decoction on intestinal flora of rats with slow transit constipation based on 16S rDNA sequencing
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摘要 目的基于16S rDNA测序分析慢传输型便秘(STC)脾虚证小鼠肠道菌群结构特征,初步探讨枳术丸对STC肠道菌群的可能干预机制。方法40只小鼠随机分成正常组、模型组、枳术丸组与莫沙必利组。造模组(模型组、枳术丸组与莫沙必利组)采用番泻叶灌胃,随后控制饮食饮水采用饥饱失常的方法造成脾虚便秘小鼠模型。造模成功后,枳术丸组给予中药水煎剂灌胃,莫沙必利组给予莫沙必利悬浊液灌胃,正常组与模型组给予蒸馏水灌胃。连续给药7 d后测定小鼠肠道推进率和血清D−木糖水平,采集小鼠结肠内粪便进行16S rDNA检测,分析样本菌群的多样性与丰度,分析门、属、种水平的物种组成。结果模型组肠道菌群丰富度指数(Chao1指数)与多样性指数(Shannon指数)较正常组均显著降低(P<0.05),说明STC发病过程中伴随肠道菌群物种丰富度水平和菌群物种数目的降低以及菌群多样性水平的降低。在门水平上,正常组与模型组均以厚壁菌门(Firmicutes)和拟杆菌门(Bacteroidetes)为主;在属水平上,模型组肠鼠杆菌属(Muribaculum)、拟杆菌属(Bacteroides)、乳杆菌属(Lactobacillus)和粪杆菌属(Faecalibaculum)丰度显著升高(P<0.05),Lachnoclostridium和艾森伯格菌属(Eisenbergiella)丰度降低(P<0.05);在种水平上,模型组产酸拟杆菌(Bacteroides acidifaciens)、Faecalibaculum rodentium和约氏乳酸杆菌(Lactobacillus johnsonii)丰度较正常组显著升高(P<0.05),Eisenbergiella sp.丰度显著降低(P<0.05)。以上提示STC脾虚证小鼠肠道菌群多样性发生改变。经枳术丸治疗后,枳术丸组肠鼠杆菌属、拟杆菌属、乳杆菌属、粪杆菌属、Faecalibaculum rodentium和约氏乳酸杆菌丰度显著降低(P<0.05),Lachnoclostridium、艾森伯格菌属和Eisenbergiella sp.丰度升高(P<0.05)。结论枳术丸对STC脾虚证小鼠肠道菌群多样性改变有明显的调节作用,并且其作用机制与调节特定菌群有关。 Objective To analyze the structural characteristics of intestinal flora in mice with slow transit constipation(STC)based on 16S rDNA sequencing and explore the possible intervention mechanism of Zhizhuwan Decoction(ZWD).Methods Forty mice were randomly divided into the normal group,model group,ZWD group and Mosapride group;the modeling groups(model group,ZWD group and Mosapride group)were gavaged with senna leaf,followed by controlled diet and water to establish mouse models of spleen deficiency constipation with hunger and satiety method.The intestinal propulsion rate and serum D-xylose level of mice were measured after 7 days of continuous administration,and faeces were collected from the colon for 16S rDNA analysis.Results The indexes Chao1 and Shannon were significantly lower in the model group compared to the normal group(P<0.05),indicating that the pathogenesis of STC was accompanied by a decrease in the richness and number of intestinal flora species as well as an increase in the level of flora diversity.At the phylum level,Firmicutes and Bacteroidetes were dominant in both the normal and model groups.At the genus level,Muribaculum,Bacteroides,Lactobacillus and Faecalibaculum were significantly more abundant in the model group(P<0.05),while Lachnoclostridium and Eisenbergiella were less productive(P<0.05).At the species level,Bacteroides acidifaciens,Faecalibaculum rodentium and Lactobacillus johnsonii were significantly more abundant in the model group compared to the normal group(P<0.05),and Eisenbergiella sp.was substantially less productive(P<0.05).This showed that the intestinal flora diversity of the mice with STC and spleen deficiency is altered.After treatment with Zhizhuwan,the abundances of Muribaculum,Bacteroides,Lactobacillus,Faecalibaculum,Faecalibaculum rodentium and Lactobacillus johnsonii significantly reduced(P<0.05),and those of Lachnoclostridium,Eisenbergiella and Eisenbergiella sp.increased in the ZWD group(P<0.05).Conclusion Zhizhuwan has a significant modulating effect on the intestinal flora diversity in mice with STC spleen deficiency,and its mechanism of action is related to the regulation of specific flora.
作者 宋子颛 刘富林 张宇娟 张婷 夏旭婷 施敏 SONG Zi-zhuan;LIU Fu-lin;ZHANG Yu-juan;ZHANG Ting;XIA Xu-ting;SHI Min(Department of Basic Theory of Chinese Medicine,School of Chinese Medicine,Hu’nan University of Chinese Medicine,Changsha,Hu’nan 410000,China;不详)
出处 《中国微生态学杂志》 CAS CSCD 2023年第4期414-419,共6页 Chinese Journal of Microecology
基金 湖南省自然科学基金(2022JJ30437) 湖南省自然科学基金(2020JJ5425) 湖南省教育厅科学研究一般项目(19C1865)。
关键词 枳术丸 慢传输型便秘 肠道菌群 16S rDNA Zhizhuwan Slow transit constipation Intestinal flora 16S rDNA
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