摘要
为获得具有体外抗氧化和降血脂活性的黄粉虫酶解产物,文章以黄粉虫脱脂粉为原料,利用响应面分析法优化酶解工艺,并对其活性组成和体外活性进行研究。结果表明,在温度60℃、液料比30 mL/g、加酶量4800 U/g、pH10、酶解3 h条件下,酶解产物DPPH自由基消除率最高。该酶解产物中含有蛋白类物质738.17 mg/g、多糖59.42 mg/g、还原糖31.01 mg/mL、黄酮6.93 mg/g。酶解产物浓度为8 mg/mL时,对DPPH自由基、超氧阴离子、羟基自由基消除率最高,分别为76.53%、22.08%和48.73%;IC_(50)值分别为2.13、27.50 mg/mL和9.61 mg/mL,对甘氨胆酸钠结合量最大为0.055μmol/mg。研究表明黄粉虫酶解产物具有一定的体外抗氧化活性和结合甘氨胆酸钠能力,为黄粉虫酶解物在降血脂方面的应用提供了理论依据。
In order to obtain the enzymatic digestion product of Tenebrio molitor(L.)with in vitro antioxidant and hypolipidemic activities,this article uses Tenebrio molitor(L.)defatted powder as raw material,optimizes the enzymatic digestion process using response surface analysis,and investigates its active composition and in vitro activity.The results showed that the DPPH radical elimination rate was the highest under the conditions of 60℃,30 mL/g liquid to material ratio,4800 U/g enzyme addition,pH10,and 3 h enzymatic hydrolysis.The hydrolysate contains 738.17 mg/g protein,59.42 mg/g polysaccharide,31.01 mg/mL reducing sugar,and 6.93 mg/g flavone.When the concentration of hydrolysate was 8 mg/mL,the elimination rate of DPPH radical,superoxide anion and hydroxyl radical was the highest,up to 76.53%,22.08%and 48.73%respectively;IC_(50) values were 2.13,27.50 mg/mL,and 9.61 mg/mL,respectively;and the maximum binding amount of sodium glycocholate was 0.055μmol/mg.The study showed that the hydrolysates of Tenebrio molitor(L.)had certain antioxidant activity in vitro and ability to bind sodium glycylcholic acid,which provided a theoretical basis for the application of Tenebrio molitor(L.)hydrolysates in reducing blood lipids.
作者
李玉婷
张天宝
杜慧玲
郭继虎
张鸣慧
亢芬
张佳乐
LI Yuting;ZHANG Tianbao;DU Huiling;GUO Jihu;ZHANG Minghui;KANG Fen;ZHANG Jiale(Foundation Department of Shanxi Agricultural University,Taigu 030801,China)
出处
《食品科技》
CAS
北大核心
2023年第4期245-251,共7页
Food Science and Technology
基金
山西省重点研发计划重点项目(201603D21107-1,201703D221025-3,201803D221011-11)。
关键词
黄粉虫酶解物
制备
抗氧化
胆酸盐
enzymatic hydrolysate of Tenebrio molitor(L.)
preparation
antioxidation
bile salt
