摘要
目的 观察敛疮生肌灌肠疗法对溃疡性结肠炎大鼠黏膜修复及结肠组织中白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、核转录因子κBp65(NF-κBp65)、细胞间黏附分子-1(ICAM-1)表达的影响。方法 选取10只SD大鼠作为正常组,另取20只SD大鼠建立溃疡性结肠炎模型。将造模成功大鼠随机分为模型组和敛疮生肌组,每组10只。敛疮生肌组给予0.45 g/mL的敛疮生肌方浓缩水煎液2 mL灌肠,模型组、正常组给予等量生理盐水灌肠,均1次/d,连续灌肠7 d。根据大鼠体重、大便性状、便血情况计算DAI评分;HE染色观察结肠组织病理形态,计算结肠组织病理评分;ELISA法检测结肠组织中IL-6、IL-10含量,免疫组化法检测NF-κBp65蛋白表达情况,RT-PCR法检测ICAM-1 mRNA表达情况。结果 模型组DAI评分、结肠组织病理学评分及结肠组织中IL-6含量、NF-κBp65蛋白表达光密度值、ICAM-1mRNA表达量均明显高于正常组(P均<0.05),结肠组织中IL-10含量明显低于正常组(P<0.05);敛疮生肌组DAI评分、结肠组织病理学评分及结肠组织中IL-6含量、NF-κBp65蛋白表达光密度值、ICAM-1 mRNA表达量均明显低于模型组(P均<0.05),结肠组织中IL-10含量明显高于模型组(P<0.05)。结论 敛疮生肌灌肠疗法可以有效减轻溃疡性结肠炎大鼠肠道免疫炎症反应,促进肠黏膜修复,其机制可能与上调IL-10表达和下调IL-6、NF-κBp65、ICAM-1的表达有关。
Objective It is to observe the effects of enema therapy for constraining sores and promoting tissue regeneration on mucosal repair and expression of interleukin-6(IL-6),interleukin-10(IL-10),nuclear transcription factorκBp65(NF-κBp65)and intercellular adhesion molecule-1(ICAM-1)in colonic tissues of rats with ulcerative colitis.Methods Ten SD rats were selected as the normal group,and another 20 SD rats were taken to establish the models of ulcerative colitis.The successfully modeled rats were randomly divided into model group and group of therapy for constraining sores and promoting tissue regeneration,10 rats in each group.The group of therapy for constraining sores and promoting tissue regeneration was given concentrated water decoction for constraining sores and promoting tissue regeneration 2 mL by enema,the model group and normal group were given equal amount of normal saline by enema,both once daily,continuously treated for 7 days.The DAI score was calculated based on the body weight,stool characteristics and stool blood of the rats;the histopathological morphology of the colon was observed by HE staining and the histopathological score of the colon was calculated;the contents of IL-6 and IL-10 in the colon tissue were detected by ELISA,the expression of NF-κBp65 protein was detected by immunohistochemistry,and the expression of ICAM-1 mRNA was detected by RT-PCR.Results The DAI score,colon histopathology score and IL-6 content,NF-κBp65 protein expression optical density value and ICAM-1 mRNA expression in colon tissue in the model group were significantly higher than those in the normal group(all P<0.05),and IL-10 content in colon tissue was significantly lower than that in the normal group(P<0.05);the DAI score,colon histopathology score and IL-6 content,NF-κBp65 protein expression optical density value and ICAM-1 mRNA expression in colon tissue in the group of therapy for constraining sores and promoting tissue regeneration were significantly higher than those in the model group(all P<0.05),and IL-10 content in colon tissue was significantly lower than that in the model group(P<0.05).Conclusion Enema therapy for constraining sores and promoting tissue regeneration can effectively reduce the response of intestinal immune inflammation and promote intestinal mucosal repair in rats with ulcerative colitis.The mechanism may be related to upregulating the expression of IL-10 and downregulating expression of IL-6,NF-κBp65,and ICAM-1.
作者
刘竺华
李立娟
任顺平
李宝乐
郝海蓉
李佩芳
张小强
陈翩
LIU Zhuhua;LI Lijuan;REN Shunping;LI Baole;HAO Hairong;LI Peifang;ZHANG Xiaoqiang;CHEN Pian(Affiliated Hospital of Shanxi University of Traditional Chinese Medicine,Taiyuan 030024,Shanxi,China;Shanxi University of Traditional Chinese Medicine,Taiyuan 030024,Shanxi,China;Shanxi Acupuncture Hospital,Taiyuan 030024,Shanxi,China;Wuhan Servicebio Technology Co.,Ltd.,Wuhan 430000,Hubei,China)
出处
《现代中西医结合杂志》
CAS
2023年第8期1058-1062,共5页
Modern Journal of Integrated Traditional Chinese and Western Medicine
基金
山西中医药大学创新能力培育计划项目(2019PY-004)
山西省基础研究计划项目(20210302123225)。
关键词
溃疡性结肠炎
敛疮生肌灌肠疗法
白细胞介素-6
白细胞介素-10
细胞间黏附分子-1
核转录因子κBp65
ulcerative colitis
enema therapy for constraining sores and promoting tissue regeneration
interleukin-6
interleukin-10
nuclear transcription factorκBp65
intercellular adhesion molecule-1
