摘要
目的:建立高效液相色谱法测定复方益康宁片的含量。方法:采用HPLC法,ODS柱,以甲醇-0.06％庚烷磺酸钠溶液(含1.2％冰醋酸与0.12％三乙胺)(25:75)为流动相,检测波长290nm。结果:盐酸普鲁卡因在4.3～51.7μg·ml^(-1)范围内有良好的线性关系(r=0.999 9),平均回收率为100.2％,维生素B_6在1.2～14.2 ug·ml^(-1)范围内有良好的线性关系(r=0.999 8),平均回收率为100.5％。结论:本法快速,简便准确。
Objective: To determine of content in compound procaine hydrochloride and vitamin B6 tablets by HPLC. Method: ODS column was used, menthanol-0.6% sodium heptunesulfonate solution(containing 1.2% acetic acid glacial and 0.12% triethy-lamine)were served as mobile phase, it was detected at wavelength 290 nm by UV-detector. Result: Procaine hydrochloride presented a good linear relation within a range of 4.3-51.7 ?g. mL-1, an average recovery was 100.2% . Vitamin B6 presented a good linear relation within a range of 1.2-14.2 ?g. mL-1, the average recovery was 100.5 %. Conclusion; This method is rapid, simple, and accurate.
出处
《中国药师》
CAS
2002年第11期662-663,共2页
China Pharmacist
