摘要
广藿香醇是存在于中药广藿香挥发油中的一种重要倍半萜类化合物,被认为是广藿香油药理功效和香味的主要贡献成分,具有抗菌、抗肿瘤和抗氧化等多种生物学活性。目前,全球广藿香醇及其混合精油需求量大,传统植提法存在浪费土地,污染环境等诸多问题,因而亟需一种新的方法高效、低成本的生产广藿香醇。为了拓宽广藿香醇的生产方式,实现在酿酒酵母中异源生产广藿香醇,该研究首先将来源于广藿香Pogostemon cablin的广藿香醇合酶(PS)基因进行密码子优化后置于诱导型强启动子GAL1下,转入萜类底盘菌YTT-T5,成功获得可发酵生产(4.0±0.3)mg·L^(-1)广藿香醇的工程菌PS00。为了提高转化率,采用了蛋白质融合手段将丹参来源的SmFPS基因与PS基因进行融合,广藿香醇摇瓶产量提高25倍,达(100.9±7.4)mg·L^(-1)。在此基础上,进一步优化融合基因的拷贝数,广藿香醇的产量提升了90%,达到(191.1±32.7)mg·L^(-1)。最终通过发酵工艺优化,该菌在精确发酵体系中广藿香醇产量能达到2.1 g·L^(-1),为目前报道最高水平。该研究为广藿香醇的绿色生产提供了重要基础。
Patchoulol is an important sesquiterpenoid in the volatile oil of Pogostemon cablin,and is also considered to be the main contributing component to the pharmacological efficacy and fragrance of P.cablin oil,which has antibacterial,antitumor,antioxidant,and other biological activities.Currently,patchoulol and its essential oil blends are in high demand worldwide,but the traditional plant extraction method has many problems such as wasting land and polluting the environment.Therefore,there is an urgent need for a new method to produce patchoulol efficiently and at low cost.To broaden the production method of patchouli and achieve the heterologous production of patchoulol in Saccharomyces cerevisiae,the patchoulol synthase(PS)gene from P.cablin was codon optimized and placed under the inducible strong promoter GAL1 to transfer into the yeast platform strain YTT-T5,thereby obtaining strain PS00 with the production of(4.0±0.3)mg·L^(-1) patchoulol.To improve the conversion rate,this study used protein fusion method to fuse SmFPS gene from Salvia miltiorrhiza with PS gene,leading to increase the yield of patchoulol to(100.9±7.4)mg·L^(-1) by 25-folds.By further optimizing the copy number of the fusion gene,the yield of patchoulol was increased by 90%to(191.1±32.7)mg·L^(-1).By optimizing the fermentation process,the strain was able to achieve a patchouli yield of 2.1 g·L^(-1) in a high-density fermentation system,which was the highest yield so far.This study provides an important basis for the green production of patchoulol.
作者
郭爽
王冬
杨婷婷
李文豪
李荣生
张国伟
张学礼
戴住波
GUO Shuang;WANG Dong;YANG Ting-ting;LI Wen-hao;LI Rong-sheng;ZHANG Guo-wei;ZHANG Xue-li;DAI Zhu-bo(College of Traditional Chinese Medicine,Hebei University,Baoding 071002,China;Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin 300308,China;Key Laboratory of Systems Biology,Chinese Academy of Sciences,Tianjin 300308,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2023年第9期2316-2324,共9页
China Journal of Chinese Materia Medica
基金
国家重点研发计划项目(2020YFA0908000)
合成生物学海河实验室颠覆性创新(人才)类项目(22HHSWSS00026)。
关键词
广藿香醇
酿酒酵母
精确发酵
patchoulol
Saccharomyces cerevisiae
high-density fermentation
