摘要
为了研究微生物菌剂对连作地块草莓(Fragaria×ananassa Duch.)生长和土壤性状的影响,设置3个处理:不施用微生物菌剂(CK)、施用单一微生物菌剂(T1)和施用复合微生物菌剂(T2),统计草莓存活率和产量,并测定土壤样品的理化性状和微生物群落结构变化。结果表明,微生物菌剂处理有助于草莓的生长和增产。与CK相比,T1、T2的草莓存活率分别提高17.99和25.19个百分点,草莓产量分别增加30.16%和41.79%。微生物菌剂处理有效改善了土壤养分。与CK相比,T2土壤有效磷和速效钾含量显著提高18.31%和55.27%。然而,微生物菌剂处理提高了土壤电导率并降低了土壤pH值,长期施用微生物菌剂存在土壤盐渍化和酸化的风险。微生物群落结构分析结果表明,草莓土壤中细菌和真菌的优势菌门分别为厚壁菌门和子囊菌门,优势菌属分别为芽孢杆菌属和曲霉属。微生物菌剂施用提高了土壤真菌群落的丰富度和多样性,但对土壤细菌群落影响较小。与CK相比,微生物菌剂处理增加了芽孢杆菌属、根霉属和假霉样真菌属的相对丰度,其中根霉属和假霉样真菌属相对丰度在T2达到显著水平;降低了鞘氨醇单胞菌属、曲霉属和青霉属的相对丰度,其中T2处理曲霉属和青霉属的相对丰度显著降低14.62和1.79个百分点。冗余分析结果表明,微生物菌剂特别是复合微生物菌剂对土壤理化性状和微生物群落结构具有明显的改善作用,有利于增加草莓的存活率和产量。本研究结果为草莓产业的可持续发展提供了生防材料和理论依据。
To explore effects of microbial agents on strawberry(Fragaria×ananassa Duch.)growth and soil properties,three treatments were set up in this study:without microbial agent(CK),with single microbial agent(T1)and with compound microbial agent(T2).Strawberry survival rate and yield were calculated,and soil samples were collected to measure soil physico chemical traits and microbial community structure.The results showed that application of microbial agents significantly improved the growth and yield of strawberry.Compared with CK,the treatments of T1 and T2 increased the strawberry survival rate by 17.99 and 25.19 percentage points,respectively,and increased strawberry yield by 30.16%and 41.79%,respectively.The microbial agents significantly improved soil fertility.Compared with CK,T2 treatment significantly increased soil available phosphorus and available potassium content by 18.31%and 55.27%,respectively.However,microbial agents enhanced soil electrical conductivity and lowered soil pH,which indicates long-term application of microbial agent may raise the risk of soil salinization and acidification.The results of microbial community structure showed that Firmicutes and Ascomycota were the dominant bacteria and fungi at phylum level,and Bacillus and Aspergillus were the dominant bacteria and fungi at genus level.Both two types of microbial agents increased the richness and diversity of soil fungal community,but had a negligible effect on the soil bacterial community.Compared with CK,the microbial agents increased the relative abundance of Bacillus,Rhizopus and Pseudallescheria,in which the relative abundance of Rhizopus and Pseudallescheria reached a significant level in T2 treatment.The microbial agents decreased the relative abundance of Sphingomonas,Aspergillus and Penicillium,while the relative abundances of Aspergillus and Penicillium were significantly decreased by 14.62 and 1.79 percentage points in T2 treatment,respectively.The results of redundancy analysis show that microbial agents,especially compound microbial agents,can significantly improve soil physico chemical properties and microbial community,which is beneficial to reduce the occurrence of diseases and increase the yield of strawberry.This study provided biocontrol materials and theoretical basis for the sustainable development of the strawberry industry.
作者
杨肖芳
郭瑞
姚燕来
朱为静
洪磊东
洪春来
朱凤香
王卫平
YANG Xiaofang;GUO Rui;YAO Yanlai;ZHU Weijing;HONG Leidong;HONG Chunlai;ZHU Fengxiang;WANG Weiping(Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou,Zhejiang 310021;Institute of Environmental,Resources and Soil fertilizer,Zhejiang Academy of Agricultural Sciences,Hangzhou,Zhejiang 310021;Xianghu Laboratory,Hangzhou,Zhejiang 311231)
出处
《核农学报》
CAS
CSCD
北大核心
2023年第6期1253-1262,共10页
Journal of Nuclear Agricultural Sciences
基金
浙江省重点研发计划(2020C02001)
大宗蔬菜产业体系-栽培与土肥研究室-产地环境综合治理科学家岗位项目(CARS-25-A-04)
院地合作项目(TZ2018018)
省三农九方(2021SNLF009)。
关键词
草莓
微生物菌剂
产量
养分
微生物群落
strawberry
microbial agent
yield
fertility
microbial community
