摘要
为获得垫状点地梅(Androsace tapete)垫状结构中的耐冷纤维素分解菌,以3个地区不同生存状态的垫状点地梅地上部分为材料,采用纯培养方法分离和纯化可培养微生物,并在4℃和15℃条件下分别用CMC固体培养基和CMC-刚果红固体培养基筛选出耐冷纤维素分解菌,对筛出的菌株进行16S rRNA和ITS区域序列的分子鉴定。结果表明,实验共获得52株14属32种耐冷纤维素分解菌,包括3种细菌、9种酵母菌和20种丝状真菌。耐冷纤维素分解菌的相对纤维素酶活性较高,代表菌株有楸子茎点霉(Didymella pomorum)、出芽短梗霉(Aureobasidium pullulans)、戈茨青霉(Penicillium goetzii)、灰玫瑰青霉(Penicillium griseoroseum)。来源于不同垫状点地梅个体之间以及叶际和内生的耐冷纤维素分解菌之间物种组成差别较大,但相对纤维素酶活性无显著差异。这些耐冷纤维素分解菌可以有效分解垫状结构中的凋落物,促进碳循环,在垫状点地梅适应极端环境并行使生态系统工程师功能中发挥重要作用,活性较高的菌株可以为寒冷地区生物降解纤维素的工业应用提供材料。
In order to obtain cold-tolerant cellulolytic strains in the cushion structure of the Androsace tapete,the above-ground parts of the Androsace tapete from three regions with different survival states were used as materials,and the culturable microorganisms were isolated and purified by pure culture method.The cold-tolerant cellulolytic strains were screened by CMC solid medium and CMC-Congo Red solid culture at 4℃and 15℃,respectively,and the strains were screened for molecular identification of 16S rRNA and ITS region sequences.The results showed that a total of 52 strains,14 genera and 32 species of cold-tolerant cellulolytic strains were obtained,including 3 species of bacteria,9 species of yeast and 20 species of filamentous fungi.The relative cellulase activities of the obtained cold-tolerant cellulolytic strains were high,and the representative strains were Didymella pomorum,Aureobasidium pullulans,Penicillium goetzii and Penicillium griseoroseum.The species composition of cold-tolerant cellulolytic strains differed significantly between individuals from different Androsace tapete and between Phyllospheric and endophytic cellulolytic strains,but there were no significant differences in relative cellulase activity.These cold-tolerant cellulolytic strains can effectively decompose litter in cushion structure,promote carbon cycling,and play an important role in the adaptation of Androsace tapete to extreme environments and perform ecosystem engineer functions,with the more active strains aiming to provide material for industrial applications of biodegradable cellulose in cold regions.
作者
朱攀
薛文凯
刘星
ZHU Pan;XUE Wenkai;LIU Xing(Laboratory of Extreme Environment Biological Resources and Adaptive Evolution,Research Center for Ecology,College of Science,Tibet University,Lhasa 850000,China;College of Life Sciences,Wuhan University,Wuhan 430072,China)
出处
《生物学杂志》
CAS
CSCD
北大核心
2023年第3期48-55,共8页
Journal of Biology
基金
西藏大学2020级硕士研究生“高水平人才培养计划”项目(2020-GSP-S047)
西藏自治区科技计划中央引导地方项目(XZ202001YD0028C)
资源与水电工程科学国家重点实验室开放课题项目(2018NSG02)
国家民委生物技术综合实验室开放课题项目(BTEC201903)
西藏自治区科技计划项目重点研发及转化计划项目(XZ202001ZY0016N)
西藏特色生物种质资源保护与基因技术开发研究项目(XZ202001ZY0016N)。
关键词
青藏高原
垫状点地梅
耐冷菌株
纤维素分解功能菌
极端环境
Qinghai-Tibetan Plateau
Androsace tapete
cold-tolerant strain
cellulolytic functional strain
extreme environment
