摘要
木霉属真菌(Trichoderma spp.)是一类有益微生物,不仅能促进植物生长,还能诱导植物对生物和非生物胁迫产生耐受性。实验室前期分离获得一株来自南极海洋沉积物的菌株TCS007,并鉴定其为棘孢木霉Trichoderma asperellum,研究表明,TCS007可显著提高植物抗盐、抗寒能力。本文以蚕豆为试材,从生理生化水平上研究了TCS007在诱导植株抗旱性方面的作用。采用温室盆栽法对不同处理条件下蚕豆的各项生理生化指标进行了测定。结果表明:在干旱胁迫下,经TCS007孢子悬浮液处理的蚕豆幼苗的株高、株重、根鲜重和总叶绿素含量相较于空白对照(CK)分别增加了28.7%、42.9%、34.2%和26.2%;叶片中的总糖、总蛋白含量和相对含水量在不同的时间点分别增加了11.1%~106.0%、38.0%~68.8%和7.0%~19.0%;蚕豆根、茎和叶中超氧化物歧化酶(SOD)活性分别增加了28.8%、13.3%和8.0%,过氧化物酶(POD)活性分别增加了10.8%、22.4%和14.8%,丙二醛(MDA)含量分别降低了37.2%、14.7%和12.1%;此外,蚕豆根、茎和叶中植物激素脱落酸(ABA)含量在不同的时间点分别增加了21.1%~33.2%、44.9%~60.6%和28.2%~78.7%,生长素吲哚乙酸(IAA)含量分别增加了20.9%~29.0%、19.7%~37.3%和11.1%~23.8%。由此可见,用TCS007孢子悬浮液处理可以缓解干旱胁迫对蚕豆生长造成的影响,从生理生化水平上提高蚕豆的抗旱能力。本研究结果可为TCS007开发成为木霉生物菌肥产品提供理论依据。
Trichoderma spp.is a class of beneficial microorganisms that not only can promote plant growth but also induce plants to develop tolerance to biological and abiotic stresses.Our laboratory obtained a bacterial strain TCS007 from Antarctic marine sediments and it is identified as a Trichoderma asperellum.Previous studies have shown that TCS007 can significantly improve the ability of plants to resist salt and cold.In this paper,the role of TCS007 in inducing plant drought resistance was studied at the physiological and biochemical levels in fava beans.The experiment was conducted using the greenhouse potting method to determine the physiological and biochemical indicators of fava beans under different treatment conditions.The results showed that when treated with TCS007 spore suspension,the height,total weight,fresh root weight and total chlorophyll content of fava beans increased by 28.7%,42.9%,34.2%and 26.2%,the total sugar,total protein content,and relative water content of fava beans leaves increased by 11.1%-106.0%,38.0%-68.8%and 7.0%-19.0%,respectively,compared with blank control(CK)under drought-stress.The activities of superoxide dismutase(SOD)increased by 28.8%,13.3%and 8.0%in the roots stems and leaves of fava beans,peroxidase(POD)activity increased by 10.8%,22.4%and 14.8%,respectively,and malondialdehyde(MDA)content decreased by 37.2%,14.7%and 12.1%,respectively.In addition,the content of plant hormone abscisic acid(ABA)in the roots,stems and leaves of fava beans increased by 21.1%-33.2%,44.9%-60.6%and 28.2%-78.7%,and the content of auxin indoleacetic acid(IAA)increased by 20.9%-29.0%,19.7%-37.3%and 11.1%-23.8%,respectively.These results suggested that TCS007 can improve the drought resistance of fava beans at physiological and biochemical levels.This work provides a theoretical basis for the development of TCS007 into a bacterial fertilizer product of Trichoderma.
作者
陈赛
倪敏姿
崔峰
陈杰
CHEN Sai;NI Minzi;CUI Feng;CHEN Jie(College of Forestry and Biotechnology,Zhejiang A&F University,National and Local Joint Engineering Laboratory for Efficient Preparation Technology of Biopesticides,Hangzhou 311300,China)
出处
《农药学学报》
CAS
CSCD
北大核心
2023年第3期657-667,共11页
Chinese Journal of Pesticide Science
基金
浙江省重点研发计划(生态安全高效果蔬病害生物防治制剂研发)(2021C02046)。
关键词
棘孢木霉TCS007
干旱胁迫
植物激素
抗氧化代谢
蚕豆
Trichoderma asperellum TCS007
drought-stressed
plant hormones
antioxidant metabolism
fava beans
