摘要
嗜吞噬细胞无形体(anaplasma phagocytophilum)是一种经蜱传播的病原体,可侵染人和动物的中性粒细胞而引起无形体病。开展精准、快速的嗜吞噬细胞无形体检测,对无形体病的早诊断、早预防、早控制等具有重要意义。目前,嗜吞噬细胞无形体感染的诊断方法主要有流行病学史、临床症状表现及实验室检测等,其中分子生物学检测技术具有特异性高、操作方法简单、快速等特点,是对该病原体检测的重要方法。我们综述了近年来常见的嗜吞噬细胞无形体分子检测技术的应用进展,如巢式PCR法,荧光定量PCR法、多重PCR法、环介导等温扩增技术以及单细胞测序技术等,为后续的嗜吞噬细胞无形体研究和发展方向提供参考依据,也为无形体病的诊断提供借鉴。
Anaplasma phagocytophilum(AP)is a tick-borne pathogen that infects human and animal neutrophils to cause anaplasmosis.Accurate and rapid detection of AP is of great significance for early diagnosis,prevention and control of anaplasmosis.At present,the clinical diagnosis methods of AP infection mainly include epidemiological history,clinical manifestations and laboratory tests,among which molecular biology detection technology is an important method for AP detection because of its high specificity,simple and rapid operation.In this paper,we will review the application progress of common molecular detection techniques of AP in recent years,such as nested PCR,fluorescent quantitative PCR,multiplex PCR amplicon sequencing,loop-mediated isothermal amplification and single-cell sequencing,etc.It provides reference for the future research and development direction,and also for the diagnosis of anaplasmosis.
作者
王珂
桂雨阳
李迅
李照杰
杨邦和
WANG Ke;GUI Yuyang;LI Xun;LI Zhaojie;YANG Banghe(School of Laboratory Medicine,Wannan Medical College,Wuhu,Anhui 241002,China)
出处
《预防医学论坛》
2023年第4期311-314,320,共5页
Preventive Medicine Tribune
基金
国家级大学生创新训练项目(202110368022)。
关键词
嗜吞噬细胞无形体
蜱传疾病
分子检测技术
应用
Anaplasma phagocytophilum
Tick-borne diseases
Molecular detection technology
Application
Prospect
