摘要
乙酰化修饰是由乙酰基转移酶、去乙酰化酶介导的可逆的蛋白质翻译后修饰。其中,乙酰基转移酶将乙酰辅酶A的乙酰基团转移至底物蛋白的氨基酸残基,而乙酰基团的去除由去乙酰化酶完成。乙酰化修饰参与许多基本生物学过程的调节作用,越来越多的研究表明,蛋白质乙酰化修饰在病原菌的致病过程中具有重要作用。病原菌,如引起非典型性肺炎的嗜肺军团菌,可以通过分泌具有乙酰基转移酶活性的效应蛋白靶向宿主细胞信号通路的关键蛋白质因子,干扰宿主细胞信号通路及免疫反应。本文主要从嗜肺军团菌的致病机制、乙酰化修饰及乙酰化修饰在病原体致病过程中的调控作用进行综述,突出已知的乙酰化毒力蛋白的例子,并讨论它们如何影响与宿主的相互作用,为理解乙酰化修饰在嗜肺军团菌致病过程中的作用机制提供参考。
Acetylation is a reversible post-translational modification of proteins mediated by acetyltransferases and deacetylase.Acetyltransferase engages in transferring the acetyl moiety of acetyl-CoA to the amino acid residue of the substrate protein,and the acetyl moiety of protein is removed by deacetylase.Acetylation is involved in the regulation of many basic biological processes,acting on histones and non-histone proteins,thus affecting a series of cellular processes such as gene transcription,regulating mRNA stability,mediating protein localization and degradation,etc.More and more studies have shown that protein acetylation plays an important role in the pathogenic process of pathogenic bacteria.Legionella pneumophila(L.pneumophila)is an intravacuolar pathogen that can cause acute pneumonia in susceptible human hosts.L.pneumophila can be airborne to infect macrophages in human lung tissue.Successful intracellular survival in hosts depends on the formation of the specialized Legionella-containing vacuolar(LCV).Upon invading host cells by phagocytosis,more than 300 different effectors are delivered into host cells via the Icm/Dot type IV system(T4SS)into host cells.These effectors hijack a variety of host cellular processes by diverse mechanisms,redirecting components of the host cell secretory pathway to remodel and maturate the LCV,and play an extremely important role in L.pneumophila survival and replication in host.Chief among them are mediated the post-translational modification that perturb host signal pathway.Among L.pneumophila effectors are quite a bit proteins that show primary amino acid sequence homology to eukaryotic GNAT family acetyltransferases or prokaryotic serine/threonine acetyltransferases.This article mainly reviews acetylation modification,the pathogenic mechanism of L.pneumophila,and the role of acetylation in the pathogenic process of the pathogen and highlights the known virulence as acetyltransferase and their role in host interaction,providing a reference for understanding the mechanism of acetylation modification in the pathogenic process of L.pneumophila.
作者
钟文红
陈涛涛
欧阳松应
ZHONG Wen-Hong;CHEN Tao-Tao;OUYANG Song-Ying(College of life Science,Fujian Normal University,Fuzhou 350117,China;Biomedical Research Center of South,Fujian Normal University,Fuzhou 350117,China)
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2023年第5期1088-1098,共11页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金(82225028,8217228)
国家重点研发计划(2021YFC2301403)资助项目。
关键词
嗜肺军团菌
乙酰化修饰
致病菌
毒力因子
Legionella pneumophila
acetylation
pathogens
virulence factors
