摘要
应用葡聚糖凝胶层析法对烟草FI蛋白进行纯化,通过SDS-PAGE、PAGE电泳进行组分鉴定及质谱分析,并对FI蛋白进行小鼠限量试验。结果表明:(1)通过热沉法得到FI粗蛋白,经过凝胶层析法分离收集到3个组分。在凝胶电泳图中,明确组分1有两个亚基,分别为55、13.6 kD,经过与标准品对比确定组分1为FI蛋白。(2)质谱分析结果中FI蛋白含有20种氨基酸及人体8种必需氨基酸,且已达到FAO/WHO的优质蛋白评分标准。(3)限量试验表明FI蛋白对小鼠的临床行为学、体重、脏器系数均影响不显著(P>0.05),说明FI蛋白对小鼠生长发育和健康没有抑制作用,属于基本无毒。本试验通过蛋白质谱对FI蛋白的氨基酸组成进行评估分析及小鼠限量试验,明确了FI蛋白的营养价值及食用安全性,为烟草FI蛋白的多用途开发利用提供理论依据。
Tobacco FI protein was purified by dextran gel chromatography,component identification and mass spectrometry were performed by SDS-PAGE and PAGE electrophoresis,and FI protein was subjected to mouse limit test.The results showed that:(1)FI crude protein was obtained by heat sink method,and 3 components were separated and collected by gel chromatography.In the gel electrophoresis diagram,it is clear that component 1 has two subunits,55 kD and 13.6 kD,respectively.After comparing with the standard,it is determined that component 1 is FI protein.(2)In the results of mass spectrometry analysis,the FI protein contains 20 amino acids and 8 essential amino acids for the human body,and it has reached the FAO/WHO high-quality protein scoring standard.(3)Limited experiments showed that FI protein had no significant effect on the clinical behavior,body weight and organ coefficient of mice(P>0.05),indicating that FI protein had no inhibitory effect on the growth and health of mice,and it was basically non-toxic.In this experiment,the amino acid composition of FI protein was evaluated and analyzed through protein profile and mouse limit test,which clarified the nutritional value and food safety of FI protein,and provided a theoretical basis for the multi-purpose development and utilization of tobacco FI protein.
作者
王胜优
李美宏
胡湘洲
姚时舣
罗钰嘉
谭艾娟
WANG Shengyou;LI Meihong;HU Xiangzhou;YAO Shiyi;LUO Yujia;TAN Aijuan(College of Life Sciences,Guizhou University/Institute of Agro-bioengineering,Guizhou University/Key laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education)/Collabora-tive Innovation Center for Mountain Ecology&Agro-Bioengineering(CICMEAB),Guiyang 550025;Guizhou To-bacco Research Institute,Guiyang 550003,China)
出处
《东北农业科学》
2023年第2期135-140,共6页
Journal of Northeast Agricultural Sciences
基金
中国烟草总公司贵州省公司科技专项(201852010064014)。
