摘要
为了解湖南省道县活禽交易市场中高致病性禽流感病毒(HPAIV)的流行态势,2022年1—12月,在1个家禽批发市场、6个城镇活禽交易市场、13个乡镇活禽交易市场开展了H5、H7亚型HPAIV专项监测,共采集家禽及市场环境样品3 148份,对其进行实时荧光定量RT-PCR检测。结果显示:H5亚型HPAIV样品阳性率为1.21%(38/3 148)、H7亚型为0.51%(16/3 148);冬春寒冷季节样品阳性率较高;阳性样品以市场环境样品居多,其中案板和污水的样品阳性率较高;城镇活禽交易市场样品阳性率(3.00%)高于乡镇交易市场(0.90%),差异有统计学意义(P <0.05)。结果表明,道县活禽市场存在H5、H7亚型HPAIV污染,以H5亚型为主,环境污染较严重,冬春寒冷季节污染风险较高。因此,需要对市场进行严格清理消毒,强化市场管理,严格活禽调运检疫,实施活禽分区分类销售,以降低活禽市场传播禽流感的风险。
In order to investigate the prevalence status of highly pathogenic avian influenza virus(HPAIV)in live poultry markets in Daoxian County,Hunan Province,special monitoring for HPAIV subtypes H5 and H7 was implemented in 1 poultry wholesale market,6 live poultry markets at town level and 13 live poultry markets at township level from January to December 2022,a total of 3148 samples were collected for test by real-time fluorescence quantitative RT-PCR.The result showed that the positive rate of subtype H5 virus was 1.21%(38/3148),and that of subtype H7 virus was 0.51%(16/3148);the positive rate was higher in winter and spring;most positive samples were from market environment,especially the cutting boards and sewage;the positive rate of samples in town markets(3.00%)was significantly higher than that in township markets(0.90%),with statistical significance(P<0.05).In conclusion,the markets were infected with subtypes H5 and H7 HPAIV,mainly subtype H5 and virus contamination in environment was serious,with higher risk in winter and spring.It was thus recommended to strictly clean and disinfect markets,strengthen market management,implement movement quarantine and classified distribution of live poultry to reduce possible risk of spreading avian influenza in live poultry markets.
作者
何淑云
肖钰
朱华
陈才
何俭绪
邹玖零
He Shuyun;Xiao Yu;Zhu Hua;Chen Cai;He Jianxu;Zou Jiuling(Daoxian Animal Husbandry and Fishery Affairs Center,Daoxian,Hunan 425300,China;Yongzhou Animal Disease Prevention and Control Center,Yongzhou,Hunan 425000,China)
出处
《中国动物检疫》
CAS
2023年第6期12-15,共4页
China Animal Health Inspection
