摘要
以紫薇叶片为材料,通过Illumina Hiseq X Ten测序平台进行高通量测序,利用MISA软件分析转录组的SSR相关信息。结果表明:测序共获得31 461条Unigene序列,总长度34 408 463 bp,其中有5 617条Unigene序列含有SSR位点,共发现SSR位点6 761个,出现频率为21.49%,平均分布距离为5.09 kb;主要的优势重复类型为二核苷酸重复和三核苷酸重复,分别占总SSR的44.95%和36.41%;AG/CT和A/T为SSR优势重复基元,出现频率分别为40.23%(2 720个)和16.57%(1120个)。分析表明,紫薇转录组中SSR类型丰富、位点出现频率高、多态性潜力高,在紫薇种质遗传多样性研究、品种鉴定、杂种鉴定等方面具有应用价值。
Illumina Hiseq X Ten sequencing platform was used to conduct high-throughput sequencing on Lagerstroemia indica leaves,and SSR related information of transcriptome was analyzed by MISA software.The results showed that a total of 31461 Unigene sequences with a total length of 34408463 bp were obtained,among which 5617 Unigene sequences contained SSR loci.A total of 6761 SSR loci were found,with a frequency of 21.49%and an average distribution distance of 5.09 kb.The dominant repeats were dinucleotide and trinucleotide,accounting for 44.95%and 36.41%of the total SSR repeats,respectively.AG/CT and A/T were SSR dominant repeat primitives,with occurrence frequencies of 40.23%(2720)and 16.57%(1120),respectively.The results showed that SSR types were abundant,loci appeared frequently,and polymorphism potential was high in the transcriptome of Lagerstroemia indica,which has application value in genetic diversity analysis,variety identification,hybrid identification and so on.
作者
秦波
韦广绥
覃杰
孙开道
秦赐梅
刘昊
黄欣
QIN Bo;WEI Guang-sui;QIN Jie;SUN Kai-dao;QIN Ci-mei;LIU Hao;HUANG Xin(Guangxi Forestry Research Institute,Nanning 530002,PRC;Guangxi Gaofeng State Owned Forest Farm,Nanning 530025,PRC;Guangxi Huangmian State Owned Forest Farm,Liuzhou 545600,PRC)
出处
《湖南农业科学》
2023年第4期1-4,共4页
Hunan Agricultural Sciences
基金
广西壮族自治区林业科学研究院基本科研业务费(林科202110号)
广西特色经济林培育与利用重点实验室自主课题(JA-22-01-04
JA-20-01-03)。
关键词
紫薇
转录组
SSR
Lagerstroemia indica
transcriptome
SSR
