摘要
为探讨复方白头翁散防治鸡白痢沙门菌的效果,并研究其抑制细菌生物被膜的机制。从临床样品中分离出鸡白痢沙门菌,对其进行药敏试验,测定其生物被膜的形成能力。体外试验中,检测复方白头翁散对分离菌株的最小杀菌浓度(MBC)、生物被膜的抑制效果、抑制细菌黏附、侵袭效果以及对相关基因sipC、rpoS、rcsC、invA、hilA、csgB、bcsA和pagC表达水平的影响。结果表明:从临床样品中分离出10株鸡白痢沙门菌,这些菌株至少对1种抗生素耐药,多重耐药率为30%,最高为5重耐药;90%分离株可形成生物被膜。复方白头翁散对9株细菌的最小杀菌浓度为62.5 mg·mL^(-1);该药可在1/2 MBC浓度使分离株黏附率下降93%,侵袭率下降36%,在1/4 MBC浓度使黏附率下降87%,但使侵袭率升高;实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)分析发现,复方白头翁散下调鸡白痢沙门菌sipC基因表达量,上调其invA、bcsA和pagC等基因表达量。这一研究说明通过调节相关基因的表达水平,复方白头翁散抑制鸡白痢沙门菌生物被膜形成,从而阻止其感染。
This study investigated the preventive and therapeutic effects of Compound pulsatilla on Salmonella pullorum,and preliminarily studied its inhibition mechanism of biofilm.Salmonella pullorum were isolated from clinical samples,its drug sensitivity test was carried out,and its biofilm forming ability was analyzed.In vitro test,the effects of Compound pulsatilla on the minimum bactericidal concentration(MBC)of S.pullorum,the inhibitory effect of biofilm formation,the effect of inhibiting bacterial adhesion and invasion and the associated genes including sipC,rpoS,rcsC,invA,hilA,csgB,bcsAand pagC,were detected.The results showed that 10 strains of Salmonella pullorum were isolated from clinical samples,this isolates were resistant to at least one antibiotic;3(30%)isolates resisted 3 or more classes of antibiotics,the highest isolate exhibited resistance to 5 antimicrobials.90%of S.pullorumformed biofilm.The minimum bactericidal concentration(MBC)of Compound pulsatilla against 9 strains were 62.5mg·mL^(-1).Compound pulsatilla could reduce the adhesion and invasion rate of the isolate by 93%and 36%at 1/2MBC concentrations.However,it reduced the adhesion rate of the isolate by 87%,and increased the invasion rate at 1/4MBC concentrations.Changes in S.pullorumafter treatment with Compound pulsatilla detected by the results of quantitative real-time PCR(qRTPCR)showed that Compound pulsatilla could down-regulate the expression of S.pullorum sipC gene,and up-regulate the genes such as invA,bcsA and pagC,and so on.Compound pulsatilla can inhibit its biofilm forming by regulating the expression of S.pullorumgene to prevent infections.
作者
赵一宁
陈新延
王丽扬
周雯
魏诗谣
王彦红
ZHAO Yining;CHEN Xinyan;WANG Liyang;ZHOU Wen;WEI Shiyao;WANG Yanhong(Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and Zoonoses/College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China)
出处
《扬州大学学报（农业与生命科学版）》
CAS
北大核心
2023年第2期47-53,共7页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
江苏高校优势学科建设工程项目(PAPD)
扬州大学大学生创新创业训练计划项目(X20200717)。
