摘要
目的:探讨小鼠视神经损伤后不同时间点的视网膜神经节细胞(RGCs)和轴突数量的动态变化,进一步评估视神经损伤程度。方法:1月龄C57BL/6J小鼠20只随机分为4组:正常对照组及视神经损伤后3、7、14 d的实验组。采用小动物显微成像系统进行眼底照相及视网膜光学相干断层成像(OCT),测量视网膜全层与神经节细胞复合体(GCC)层厚度,采用免疫荧光染色和电镜评估RGCs和轴突数量的动态变化。结果:小鼠视神经损伤后3 d组的GCC层厚度、不同区域RGCs数量与正常对照组相比,差异无统计学意义(P>0.05),轴突存活率较正常对照组降低(P<0.05)。视神经损伤后7 d组的GCC层厚度、视乳头近侧及远侧区域的RGCs数量和轴突存活率较正常对照组减少(P<0.05)。视神经损伤后14 d的GCC层厚度、不同区域RGCs数量及轴突存活率较其余各组均减少(P<0.05)。GCC层厚度、RGCs数量以及轴突存活率三者之间均呈显著正相关(P<0.01)。结论:小鼠视神经损伤早期(损伤后3 d),RGCs胞体及轴突存活率高。视神经损伤中晚期(损伤后7至14 d),随着损伤时间延长,RGCs胞体及轴突存活率逐渐降低。因此,在视神经损伤早期阶段及时干预治疗有望预防视觉功能的进一步损害。
Objective:To investigate the dynamic changes in the number of retinal ganglion cells(RGCs)and axons at different time points after optic nerve crush in mice,and to further assess the degree of optic nerve injury.Methods:Twenty 1-month-old C57BL/6J mice were randomly divided into four groups:normal control group and experimental groups at 3,7,and 14 days after optic nerve injury.Fundus photography and optical coherence tomography were performed with the small-animal microscopic imaging system,and the thickness of the total retina and ganglion cell complex(GCC)were measured.Immunofluorescence staining and electron microscopy were used to assess the dynamic changes of the number of RGCs and axons.Results:Three days after optic nerve injury,the thickness of the GCC layer and the number of RGCs in different retinal regions were not significantly different from those in the normal control group(P>0.05),but the axonal survival rate was reduced(P<0.05).The thickness of the GCC layer,the number of RGCs in the peripapillary region and the lateral region of the retina,and the axonal survival rate on day 7 post-injury were reduced,compared with normal control group(P<0.05).The thickness of the GCC layer,the number of RGCs in the per retinal region,and the axonal survival rate on day 14 post-injury decreased significantly compared with other groups(P<0.05).Besides,the thickness of the GCC layer,the number of RGCs,and the axonal survival rate were all positively correlated(P<0.001).Conclusion:During the early stage of optic nerve injury(3 days post-injury),the survival rate of RGCs and axons is high.However,the survival rate of RGCs and axons gradually decreases during the middle and late stages of optic nerve injury(7 to 14 days post-injury).Therefore,prompt intervention during the early stage of optic nerve injury may be helpful to prevent further deterioration of visual function.
作者
沈冰燕
王敏
方叶楠
代琴
谢琪琦
吴文灿
SHEN Bingyan;WANG Min;FANG Yenan;DAI Qin;XIE Qiqi;WU Wencan(School of Ophthalmology&Optometry,Biomedical Engineering,Wenzhou Medical University,Wenzhou 325035,China;Eye Hospital of Wenzhou Medical University,Wenzhou 325027,China)
出处
《温州医科大学学报》
CAS
2023年第7期532-539,共8页
Journal of Wenzhou Medical University
基金
浙江省自然科学基金项目(LY22H120009)
温州市基础性科研项目(Y20210993)
国家重点研发计划项目(2021YFA1101200)。
