摘要
目的建立黔产香榧种子高效液相色谱(HPLC)的指纹图谱及多指标含量测定方法,为黔产香榧种子的质量控制与开发提供依据。方法采用Agilent Eclipse Plus C 18色谱柱(4.6 mm×150 mm,5μm);以乙腈(A)-0.06%醋酸-水溶液(B)为流动相,梯度洗脱;流速1.0 mL/min;检测波长280 nm;柱温30℃;进样量15μL。结果20批香榧种子指纹图谱相似度均大于0.983,共确定5个共有峰,指认了没食子酸和绿原酸。对样品中的没食子酸和绿原酸2种化学成分进行含量测定与方法学考察,包含专属性、线性、精密度、重复性和稳定性,均符合要求;平均回收率在97.75%~98.82%,RSD≤1.62%。香榧种子中该2种化学成分的含量分别为33.25~97.00μg/g和1072.28~1279.56μg/g。结论本研究所建立黔产香榧种子的HPLC指纹图谱和多指标含量测定的方法准确可行,可作为黔产香榧种子质量评价的一种有效手段。
Objective To establish fingerprinting and multi-component content determination methods by high performance liquid chromatography(HPLC)of the Torreya grandis seed,and provide the scientific basis for quality control and development of Torreya grandis seed.Methods Agilent Eclipse Plus C 18 column(4.6 mm×150 mm,5μm)was used.With acetonitrile(A)-0.06%acetic acid-water solution(B)as mobile phase,gradient elution was performed.The flow rate was 1.0 ml/min.The detection wavelength was 280 nm.The column temperature was 30℃.The injection volume was 15μL.Results The similarity of 20 batches of Torreya grandis seed fingerprints were all greater than 0.983.A total of 5 common peaks were identified,and gallic acid and chlorogenic acid were identified.The methodological investigations were carried out on the determination of gallic acid and chlorogenic acid in the samples,including specificity,linearity,precision,repeatability,and stability,and all met the requirements.The average recoveries ranged from 97.75%to 98.82%,RSD≤1.62%.The contents of these two chemical components in Torreya grandis seed were 33.25-97.00μg/g and 1072.28-1279.56μg/g,respectively.Conclusion The method established by this research is accurate and feasible,and can be used as an effective means for quality control of Torreya grandis seed.
作者
田鑫
张艳
余明
曹泮相
张建永
段灿灿
Tian Xin;Zhang Yan;Yu Ming;Cao Panxiang;Zhang Jianyong;Duan Cancan(Department of Pharmaceutical Analysis,School of Pharmacy,Zunyi Medical University,Zunyi Guizhou 563099,China;Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education,Zunyi Medical University,Zunyi Guizhou 563099 China;Guizhou Wuchuan Wannianfeng Agricultural Development Co.,Ltd,Wuchuan Guizhou 564300,China)
出处
《遵义医科大学学报》
2023年第6期617-623,共7页
Journal of Zunyi Medical University
基金
遵义市优秀青年科技创新人才培养项目[NO:遵优青科(2021)3]。
关键词
香榧种子
指纹图谱
含量测定
质量控制
Torreya grandis seed
fingerprint
content determination
quality control
