摘要
通过研究准格尔旗生态修复植被油松(Pinus tabulaeformis)人工林、柠条(Caragana korshinskii)人工林、自然恢复草地和撂荒地中土壤微生态环境特征,为砒砂岩区生态修复技术提供有效补充。本研究分析了4种植被类型土壤微生态环境中微生物群落结构及微生物生物量碳、氮和磷含量、土壤养分转化相关酶活性和土壤化学性质的变化规律,探讨了土壤微生物群落结构与土壤微生态环境间的关联性,并探明不同生态修复植被类型中土壤微生境的主要影响因素。结果表明(:1)4种植被类型中油松人工林土壤溶解性有机碳最高,为4.26 mg·kg^(-1);柠条人工林土壤有效氮最高,为11.69 mg·kg^(-1);而油松人工林、柠条人工林、自然恢复草地中的土壤有效磷含量无显著差异。(2)油松人工林根际土壤养分转化相关酶活性和土壤微生物生物量碳、氮和磷均显著高于其他植被类型。(3)油松人工林的土壤真菌相对丰度最大,为31.36%;柠条人工林和撂荒地土壤革兰氏阳性菌相对丰度最大,分别为35.73%和37.27%;土壤革兰氏阴性菌的相对丰度随可利用有机碳的减少呈增加趋势,油松人工林最小(36.82%),自然恢复草地最大(42.13%)。(4)土壤真菌相对丰度与土壤养分转化相关酶均呈正相关关系;偏好易分解碳源的革兰氏阴性菌与土壤微生态环境因子的相关性较小,而革兰氏阳性菌与土壤碳转化相关指标均显著负相关。(5)冗余分析发现,碱性磷酸酶作为解释度最大的环境因子,与真菌呈极显著正相关关系,与格兰氏阳性菌呈极显著负相关关系。综上,砒砂岩区生态修复植被类型中油松人工林微生态环境的稳定性相对较高,可以作为砒砂岩区最优生态修复树种,在根际土壤微生态环境变化中碱性磷酸酶和真菌协同作用有效调控砒砂岩区的环境修复。
To provide an effective supplement for ecological restoration technology in the Arsenic sandstone area,the microecological soil environment in Pinus tabulaeformis and Caragana korshinskii plantation,naturally restored grassland,and abandoned land were studied respectively.The changes in microbial community structure and microbial biomass carbon,nitrogen and phosphorus contents,soil enzyme activities related to nutrient conversion,and soil chemical properties were analyzed in different vegetation types.The correlation between soil microbial community structure and soil microecological environment was discussed,then the main influencing factors of soil microhabitat types were explored in different ecological restoration vegetation.The results showed that:(1)The soil dissolved organic carbon(C)content of P.tabulaeformis plantation was the highest of the four vegetation types,which was 4.26 mg·kg^(-1).The soil available nitrogen(N)content of the C.korshinskii plantation was the highest,which was 11.69 mg·kg^(-1).However,there was no significant difference in soil available phosphorus(P)content among P.tabulaeformis plantation,C.korshinskii plantation and natural restored grassland.(2)The enzyme activities related to nutrient conversion and soil microbial biomass C,N and P of P.tabulaeformis plantation were significantly higher than those of other vegetation types.(3)The relative abundance of soil fungi was the highest in P.tabulaeformis plantation,which was 31.36%.The relative abundance of Gram-positive bacteria in the soil of the C.korshinskii plantation and abandoned land was the highest,which were 35.73%and 37.27%.The relative abundance of soil Gramnegative bacteria increased with the decrease of available organic C.It was the lowest in P.tabulaeformis plantation(36.82%)and the highest in the natural restored grassland(42.13%).(4)The relative abundance of soil fungi was positively correlated with enzyme activities related to nutrient conversion.Gram-negative bacteria of preferred unstable C sources were less correlated with soil microecological environmental factors.Still,Grampositive bacteria were significantly negatively correlated with the indexes of soil C conversion.(5)The Redundancy analysis showed that alkaline phosphatase was the most explainable environmental factor,and it was positively correlated with fungi and negatively correlated with Gram-positive bacteria(P<0.01).In conclusion,the stability of the microecological environment of P.tabulaeformis plantation was relatively high in the ecological restoration vegetation types of the Arsenic sandstone area,which might be used as the optimal ecological restoration tree species in the Arsenic sandstone area.The synergistic effect of alkaline phosphatase and fungi in rhizosphere soil microecological environment might effectively regulate environmental restoration in Arsenic sandstone areas.
作者
许吉康
何炎红
刘婷岩
郝龙飞
张盛晰
李赵毅
XU Jikang;HE Yanhong;LIU Tingyan;HAO Longfei;ZHANG Shengxi;LI Zhaoyi(College of Forestry,Inner Mongolia Agriculture University,Hohhot 010019)
出处
《植物研究》
CAS
CSCD
北大核心
2023年第4期531-539,共9页
Bulletin of Botanical Research
基金
内蒙古自治区科技计划项目(2022YFDZ0030,2020GG0029,2020GG0075)
内蒙古农业大学大学生科技创新基金项目(KJCX2021008)
呼和浩特市重大科技专项(2022-社-重-4-2)。
关键词
植被类型
微生物群落结构
微生物生物量
土壤酶活性
土壤有效养分
vegetation type
microbial community structure
microbial biomass
soil enzyme activity
soil available nutrient
