摘要
以金叶复叶槭(Acer negundo‘Aurea’)的金黄叶、复绿叶为试验材料,以复叶槭的绿叶为对照(CK),测定并比较3种叶片类型的叶色参数、色素含量和解剖结构,探究金叶复叶槭叶片的呈色机理,为金叶植物叶色机理的研究及栽培提供理论依据。结果表明:金黄叶叶色参数L^(*)、a^(*)、b^(*)值极显著高于复绿叶和CK(P<0.01),复绿过程中叶色参数均呈下降趋势。金黄叶叶绿素a、叶绿素b、总叶绿素、类胡萝卜素含量极显著低于复绿叶和CK,ω(类胡萝卜素)/ω(总叶绿素)为0.64,极显著高于复绿叶和CK(P<0.01),复绿过程中色素含量均呈上升趋势。3种类型叶片横切面各组织厚度、比值及排列存在差异,金黄叶叶片厚度124.51μm,显著低于CK(P<0.05),栅栏组织厚度31.26μm、栅/海为0.45,均极显著低于复绿叶和CK(P<0.01),栅栏组织和海绵组织排列混乱,而另外2种类型叶栅、海组织排列较为规则有序。金黄叶叶绿体长、宽分别为3.42μm、1.27μm,均极显著小于复绿叶和CK(P<0.01),叶绿体体积小且发育不完整、缺少类囊体垛叠。初步判断类ω(类胡萝卜素)/ω(总叶绿素)值高、叶片栅栏组织较薄、栅/海值低和叶绿体内类囊体缺少垛叠是导致金叶复叶槭叶片呈现金黄色的原因。
To explore the leaf color mechanism of Acer negundo‘Aurea’,and to provide theoretical basis for the leaf color mechanism and cultivation of golden leaf plants,the golden re-green and green leaves of A.negundo‘Aurea’were used as materials,the leaf color parameters,pigment content and anatomy structure of three leaf types were measured and compared.The results showed that the leaf color parameters L^(*),a^(*),b^(*)of the golden leaves were significantly higher than re-green leaves and CK(P<0.01).The leaf color parameters of A.negundo‘Aurea’showed a downward trend during the process of re-greening.The chlorophyll a,chlorophyll b,total chlorophyll and,carotenoid content of the golden leaves were significantly lower than re-green leaves and CK,ω(carotenoid)/ω(total chlorophyll)was 0.64,which was significantly higher than re-green leaves and CK(P<0.01),and pigment content of the golden leaves increased during the process of re-green.The cross-section structure of the three types leaves were different in the thickness,the ratio and arrangement of tissue.The thickness of golden leaves was 124.51μm,which was significantly lower than that of CK(P<0.05).The palisade tissue thickness of 31.26μm and the palisade/spongy ratio of 0.45 in the golden leaves were significantly lower than other two types of leaves(P<0.01),the palisade tissue and spongy tissue were arranged irregularly and disorderly,while the other two leaf types were arranged regularly and orderly;The chloroplast length and width were 3.42μm and 1.27μm respectively,which were significantly smaller than the re-green leaves and CK(P<0.01),the chloroplast volume was small and incomplete,lacking thylakoid stacks.The highω(carotenoid)/ω(total chlorophyll),thinner palisade tissue,lower palisade/spongy ratio and lack of stacking of thylakoid in chloroplasts were the reasons for the golden color leaves of A.negundo‘Aurea’.
作者
康莹
潘晶晶
陈建华
刘权钢
董胜君
KANG Ying;PAN Jingjing;CHEN Jianhua;LIU Quangang;DONG Shengjun(College of Forestry,Shenyang Agricultural University,Shenyang 110161)
出处
《植物研究》
CAS
CSCD
北大核心
2023年第4期591-600,共10页
Bulletin of Botanical Research
基金
“十三五”国家重点研发计划项目(SQ2019YFD100071)。
关键词
金叶复叶槭
色素含量
解剖结构
呈色机理
Acer negundo‘Aurea’
pigment content
anatomical structure
coloring mechanism
