摘要
采用超高效液相色谱-四极杆/飞行时间质谱法,建立快速筛查火锅底料中11种兴奋剂和5种生物碱类药物残留量的确证方法。用80%乙腈溶液提取样品中待测物后,以PRiME HLB柱净化,采用Kinetex F5色谱柱分离,采用多反应监测高分辨扫描模式对目标物进行定性筛查和定量分析。结果表明:16种化合物的精确质量数相对偏差小于5.0×10^(-6),线性关系良好,相关系数(R^(2))大于0.997 0;回收率范围为75.2%~105.4%,相对标准偏差为1.3%~7.4%(n=6)。检出限范围为0.05~1.0μg/kg;定量限范围为0.10~2.0μg/kg。该方法简便、快速、准确,实用性强。
A new method for rapid screening for and identification of 11 foodborne stimulants and five alkaloids in hot pot soup base seasoning by ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometry(UPLC-Q-TOF/MS)was developed.The analytes from samples were extracted with 80%aqueous acetonitrile,cleaned up on a PRiME HLB column,and separated on a Kinetex F5 column prior to qualitative screening and quantitative analysis in the high-resolution scanning mode using multiple reaction monitoring(MRM).The relative deviations of the exact mass numbers of the 16 compounds were less than 5.0×10^(-6),and the linearity of the calibration curves was good with correlation coefficient(R^(2))greater than 0.9970.The recoveries of the analytes were 75.2%–105.4%,with relative standard deviation(RSD)of 1.3%–7.4%(n=6).The limit of detection(LOD)was 0.05–1.0μg/kg,and the limit of quantitation(LOQ)was 0.10–2.0μg/kg.The developed method is simple,rapid,accurate,and practical.
作者
范力欣
马俊美
何亮娜
任晓伟
贾文轩
李强
FAN Lixin;MA Junmei;HE Liangna;REN Xiaowei;JIA Wenxuan;LI Qiang(Hebei Food Safety Key Laboratory,Key Laboratory of Special Food Supervision Technology for State Market Regulation,Hebei Engineering Research Center for Special Food Safety and Health,Hebei Food Inspection and Research Institute,Shijiazhuang 050000,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2023年第12期360-367,共8页
Food Science
基金
河北省技术创新引导计划项目(21475501D)。
关键词
超高效液相色谱
四极杆/飞行时间质谱
火锅底料
食源性兴奋剂
生物碱
定性筛查和定量分析
ultra-high performance liquid chromatography
quadrupole-time of flight mass spectrometry
hot pot soup base seasoning
foodborne stimulants
alkaloids
qualitative screening and quantitative analysis
