摘要
目的:探讨艾灸足三里对变应性鼻炎(AR)大鼠鼻黏膜免疫功能的影响。方法:将40只SD大鼠随机分为正常组、模型组、对照组和治疗组,每组10只。采用卵清蛋白致敏法制备AR大鼠模型。造模成功后对照组与治疗组进行艾灸治疗,正常组、模型组只捆绑不艾灸,疗程14 d。观察4组行为学评分,鼻黏膜组织病理情况,分泌性免疫球蛋白A(sIgA)、CD4^(+)、CD8^(+)表达水平。结果:治疗后,治疗组的行为学评分较模型组降低,差异有统计学意义(P<0.05);治疗后,正常组、治疗组嗜酸性粒细胞、鼻咽冲洗液sIgA表达及CD4^(+)、CD8^(+)水平与模型组比较,差异均有统计学意义(P<0.05或P<0.01)。结论:艾灸足三里可改善AR大鼠行为学评分及鼻黏膜组织病理情况,降低嗜酸性粒细胞数,并能下调鼻黏膜sIgA、CD4^(+)、CD8^(+)表达,这可能是其治疗AR的免疫机制之一。
Objective:To investigate the effect of moxibustion at Zusanli point on the immune function of na-sal mucosa in rats with allergic rhinitis(AR).Methods:A total of 40 Sprague-Dawley rats were randomly divided into normal group,model group,control group,and treatment group,with 10 rats in each group.Ovalbumin sensitiza-tion was used to establish a rat model of AR.After successful modeling,the rats in the control group and treatment group were given moxibustion,while those in the normal group and the model group were tied up alone without moxibustion,and the course of treatment was 14 days.The four groups were observed in terms of behavioral score,pathology of nasal mucosa,and expression levels of secretory immunoglobulin A(sIgA)and CD4^(+)and CD8^(+)cells.Results:After treatment,the treatment group had a significant reduction in behavioral score compared with the model group(P<0.05),and there were significant differences in eosinophils and the expression levels of sIgA and CD4^(+)and CD8^(+)cells in rhinopharynx flush fluid between the normal/treatment group and the model group(P<0.05 or P<0.01).Conclusion:In rats with AR,moxibustion at Zusanli point can improve behavioral score and the pathology of nasal mucosa,reduce the number of eosinophils,and downregulate the expression levels of sIgA and CD4^(+)and CD8^(+)cells in nasal mucosa,which might be one of the immune mechanisms of moxibustion in the treat-ment of AR.
作者
胡蓉
刘样
曾斯琴
李新宇
王晶
HU Rong;LIU Yang;ZENG Siqin;LI Xinyu;WANG Jing(Hunan Traditional Chinese Medical College,Zhuzhou 412012,Hunan,China)
出处
《湖南中医杂志》
2023年第5期149-153,共5页
Hunan Journal of Traditional Chinese Medicine
基金
湖南省中医药科研计划项目(201393)。
关键词
变应性鼻炎
艾灸
足三里
免疫功能
实验研究
allergic rhinitis
moxibustion
Zusanli
immune function
experimental study
