摘要
目的对青龙衣黄酮类化合物的抗氧化应激作用机制进行网络药理学分析。方法通过文献检索和在线数据库获取成分及作用靶点,利用韦恩在线分析工具将药物靶点和疾病靶点取交集;运用Cytoscape软件构建“药物-成分-靶点”网络图,依据其度值筛选出核心靶点;运用STRING数据库构建蛋白质相互作用(PPI)网络,运用DAVID数据库对关键靶点进行GO功能富集和KEGG通路分析。结果筛选出柚皮素、球松素、黄芩素、异泽兰黄素等32个活性成分;关键靶点有EGFR、MAPK1、STAT3、AKT1等,主要通过蛋白质自动磷酸化、药物反应、氧化还原过程、ATP结合等生物过程以及肿瘤信号通路、PI3K-Akt信号通路、雌激素信号通路等发挥作用。结论青龙衣黄酮类化合物通过多组分、多靶点、多通路协同发挥抗氧化应激作用。
Objective To predict the potential mechanism of antioxidant action of Qinglongyin flavonoids by network pharmacology.Methods The components and targets were obtained through literature search and online database,and the Wayne online analysis tool was used to intersect drug targets and disease targets.The"drug-component-target"network diagram was constructed by using Cytoscape software,and the core targets were screened out according to their degree values.The STRING database was used to construct a protein interaction(PPI)network,and the DAVID database was used to perform GO function enrichment and KEGG pathway analysis for key targets.Results 32 active ingredients including naringenin,coccidiotin,baicalein and isozelanxlavin were screened.The key targets are EGFR,MAPK1,STAT3,AKT1,etc,which mainly play a role through biological processes such as protein automatic phosphorylation,drug reaction,redox process,ATP binding,tumor signaling pathway,PI3K-Akt signaling pathway,estrogen signaling pathway,etc.Conclusion Qinglongyin flavonoids exert antioxidant effects through multi-component,multi-target and multi-pathway synergy.
作者
满红岩
崔新刚
孙雪
沈广志
MAN Hong-yan(School of Pharmacy,Mudanjiang Medical University,Mudanjiang 157011,China)
出处
《牡丹江医学院学报》
2023年第3期32-34,125,共4页
Journal of Mudanjiang Medical University
基金
黑龙江省省属高等学校基本科研业务费项目(2021-KYYWF-0485,2021-KYYWF-0549,2021-KYYWF-0481)
黑龙江省自然科学基金项目(LH2021B028)
牡丹江市科技局项目(HT2022JG134)。
关键词
网络药理学
青龙衣
黄酮
抗氧化应激
Network Pharmacology
Qinglongyin
flavonoids
Antioxidant
