摘要
为明确贺兰山东麓老葡萄园卷叶病发生状况及感染病毒的种类,对贺兰山东麓4个种植区5个主栽酿酒葡萄品种进行了葡萄卷叶病田间危害调查,采用RT-PCR方法对92份样品进行6种葡萄卷叶伴随病毒GLRaV-1、GLRaV-2、GLRaV-3、GLRaV-4、GLRaV-7和GLRaV-13的检测。结果表明:西夏王酒庄葡萄园的平均发病率最高,为66.43%,病情指数为41.43;志辉源石葡萄园平均发病率最低,为5.64%,病情指数为1.89。5个品种中,‘蛇龙珠’为葡萄卷叶病的易感品种,其平均发病率高达70.47%;并且酿酒葡萄品种均易被GLRaV-1、GLRaV-2和GLRaV-3卷叶伴随病毒感染,其中GLRaV-3的平均检出率最高,为70.17%;病毒的序列比对分析表明,该地区种植的葡萄品种感染了3种卷叶伴随病毒,与来源于加拿大、匈牙利、土耳其、法国、葡萄牙、意大利、巴西、希腊、中国(辽宁)和南非地区的病毒基因序列同源率较高,为95%以上。本研究为贺兰山东麓地区有效控制酿酒葡萄卷叶病的发生,减少葡萄病毒病的传播和蔓延,提供理论依据。
In order to clarify the occurrence of grapevine leafroll disease and the types of viruses in old vineyards in the eastern foot of Helan Mountains,five major wine grape varieties cultivated in four growing areas of Helan Mountains were investigated for field hazard of grapevine leafroll disease.RT-PCR was used to detect GLRaV-1,GLRaV-2,GLRaV-3,GLRaV-4,GLRaV-7 and GLRaV-13,six kinds of Grapevine leafroll-associated viruses in ninety-two samples.The results showed that the incidence of Xixiawang vineyard was the highest(66.43%),and the disease index was 41.43.The incidence of Zhihui vineyard was the lowest(5.64%),and the disease index was 1.89.'Cabernet Gernischt'grapevine was a susceptible variety of grape leafroll disease,and its average incidence reached 70.47%.Wine grape varieties were susceptible to three kinds of Grapevine leafroll-associated viruses infection,namely GLRaV-1,GLRaV-2 and GLRaV-3,among which GLRaV-3 has the highest detection rate.The sequence alignment analysis of viruses showed that the wine grape varieties grown in this region were infected by three Grapevine leafroll-associated viruses,which from different regions,and these Grapevine leafroll-associated viruses had a high homology rate of more than 95%with viral gene sequences originating in Canada,Hungary,Turkey,France,Portugal,Italy,Brazil,Greece,China(Liaoning)and South Africa.This study provides a theoretical basis for effectively controlling the occurrence of Grapevine leafroll disease and reducing the spread of Grapevine virus disease in the eastern foot of Helan Mountains.
作者
黄强
张强强
顾沛雯
HUANG Qiang;ZHANG Qiangqiang;GU Peiwen(School of Agriculture,Ningxia University,Yinchuan 750021,China)
出处
《中外葡萄与葡萄酒》
北大核心
2023年第4期40-48,共9页
Sino-Overseas Grapevine & Wine
基金
国家重点研发计划项目(2019YFD1002502)。
关键词
贺兰山东麓
酿酒葡萄
葡萄卷叶病
葡萄卷叶伴随病毒
RT-PCR检测
the eastern foot of Helan Mountains
wine grape
grapevine leafroll disease
grapevine leafroll-associated viruses
detection of RT-PCR
