摘要
为探究外源NaHS对盐胁迫下赤苍藤(Erythropalum scandens)幼苗的调节作用,为赤苍藤苗期及成年期栽培、抗盐性育种提供参考,以2年生赤苍藤扦插苗为对象,分析NaCl胁迫(100和200 mmol/L)下,添加不同梯度NaHS对赤苍藤幼苗光合及生理特性的影响。结果表明,NaCl胁迫严重影响赤苍藤幼苗光合和生理状况。NaCl胁迫下,赤苍藤幼苗叶片的叶绿素a、叶绿素b含量均显著低于CK,叶肉细胞中游离脯氨酸大量积累;植株光合作用受到显著影响,整体弱于CK。高浓度(200 mmol/L)NaCl胁迫对赤苍藤幼苗造成的影响总体高于低浓度(100 mmol/L)。添加适当浓度的NaHS(≤50μmol/L)对NaCl胁迫有一定缓解作用,可增加叶绿素a、叶绿素b含量,提升光合作用,减少游离脯氨酸积累。但NaHS释放出的H_(2)S有一定毒性,高浓度NaHS(>50μmol/L)的叶面施用可能对赤苍藤幼苗叶片细胞产生毒害,影响其光合作用、叶绿素合成等进程,导致生长受到影响。隶属度分析结果表明,添加NaHS可通过增加叶片叶绿素含量、提高叶片光合细胞活性及调节细胞内渗透物质含量等途径缓解盐胁迫对赤苍藤幼苗造成的伤害,提高耐盐能力;其中,添加20μmol/L NaHS缓解效果最佳。
In order to explore regulatory effects of exogenous NaHS on Erythropalum scandens seedlings under salt stress,and provide reference for cultivation of E.scandens at seedling stage,adult stage,and salt resistance breeding,taking two-year-old cutting seedlings of E.scandens as objects,effects of NaHS with different con⁃centrations on photosynthetic and physiological characteristics of E.scandens seedlings under NaCl(100 and 200 mmol/L)stress were analyzed.Results showed that NaCl stress had serious effects on photosynthetic and physiological status of E.scandens seedlings.Under NaCl stress,contents of chlorophyll a and chlorophyll b in leaves of E.scandens seedlings were significantly lower than those of CK,and free proline in mesophyll cells was largely accumulated.Photosynthesis of plants was significantly affected,which was weaker than CK.Effects of high concentration(200 mmol/L)of NaCl stress on E.scandens seedlings were generally higher than those of low concentration(100 mmol/L).Suitable concentration of NaHS(≤50μmol/L)had certain relieved effect for NaCl stress,which could improve chlorophyll a content,chlorophyll b content and photosynthesis,and reduce accumulation of free proline.While,H_(2)S released by NaHS had certain toxicity.Leaf application of high concen⁃tration of NaHS(>50μmol/L)might cause toxicity to leaf cells of E.scandens seedlings,and affected processes of photosynthesis and chlorophyll synthesis,which could have negative effects on growth.Results of member⁃ship analysis indicated that application of exogenous NaHS could alleviate damage of salt stress to E.scandens seedlings by increasing chlorophyll content of leaves,improving activity of leaf photosynthetic cells,and regulat⁃ing contents of intracellular osmotic substances to improve salt tolerance.The optimal concentration of NaHS was 20μmol/L.
作者
廖醒
覃凌薇
吴志培
马道承
王凌晖
Liao Xing;Qin Lingwei;Wu Zhipei;Ma Daocheng;Wang Linghui(Nanning Arboretum,Nanning,Guangxi 530031,China;College of Forestry,Guangxi University,Nanning,Guangxi 530004,China)
出处
《广西林业科学》
2023年第3期324-330,共7页
Guangxi Forestry Science
基金
广西林业科技项目(桂林科研[2021]16号)
校企合作项目(南宁树木园科字2018-1号)。
关键词
森林培育
赤苍藤
NACL胁迫
NaHS
光合特性
生理特性
隶属度分析
silviculture
Erythropalum scandens
NaCl stress
NaHS
photosynthetic characteristic
physiologi⁃cal charateristict
membership analysis
