摘要
目的利用16对微卫星引物分析采自内蒙古自治区(内蒙古)3个样地(新巴尔虎右旗、东乌珠穆沁旗和锡林浩特市)共9批布氏田鼠样本群的遗传多样性。方法对2021年5、7、9月采用鼠夹法和鼠笼法采集的275只布氏田鼠用磁珠法提取DNA,用16对荧光标记的微卫星引物做PCR扩增,用GeneMarker软件获取微卫星数据,用GenAIEx 6.5、Arlequin(version 30)、structure 2.3.4等生物信息软件分析布氏田鼠的种群遗传多样性及种群遗传结构。结果16对微卫星引物共检测到472个等位基因,多态信息含量平均值为0.8213,香农信息指数平均值为1.812,观察杂合度波动区间为0.478~0.951,期望杂合度的波动区间为0.575~0.891,聚类分析及主成分分析表明均可以将9个布氏田鼠样本群主要按照月份划分为4个类群,4个类群的遗传多样性指标5月>7月>9月,分子方差分析表明布氏田鼠个体间的变异(88.51%)远大于类群间(5.04%)和种群间(6.45%)的变异。Mantel test结果显示布氏田鼠遗传距离和地理距离无显著相关性(R2=0.0016)。结论此次采集的内蒙古地区布氏田鼠呈较高的遗传多样性,布氏田鼠种群的遗传变异主要存在于个体间,季节变化可能是影响布氏田鼠种群遗传结构的主要因素。
Objective To analyze the genetic diversity of nine batches of Lasiopodomys brandtii rodents from three sample plots(New Barag Right banner,East Ujimqin banner,and Xilinhot city)of the Inner Mongolia Autonomous Region(Inner Mongolia),China by using 16 pairs of microsatellite primers.Methods A total of 275 L.brandtii rodents were trapped using snaps or cages in May,July,and September of 2021.DNA was extracted from them with magnetic beads,followed by PCR amplification with 16 pairs of fluorescent-labeled microsatellite primers.The microsatellite data were obtained using GeneMarker.The genetic diversity and structure of L.brandtii populations were analyzed using GenAIEx 6.5,Arlequin(version 30),and structure 2.3.4.Results A total of 472 alleles were detected by using 16 pairs of microsatellite primers.The average polymorphic information content was 0.8213.The average Shannon’s information index was 1.812.Observed heterozygosity ranged from 0.478 to 0.951.Expected heterozygosity ranged from 0.575 to 0.891.According to cluster analysis and principal component analysis,the nine L.brandtii populations could be divided into four groups by month.The genetic diversity index of the four groups was May>July>September.The analysis of molecular variance showed that the variation among individuals(88.51%)was much greater than that among the groups(5.04%)and that among the populations(6.45%).The mantel test showed no significant correlation between genetic distance and geographical distance of L.brandtii(R2=0.0016).Conclusions The L.brandtii populations sampled in Inner Mongolia had high genetic diversity.The genetic variation of L.brandtii populations was mainly observed among individuals.Seasonal variation could be the main factor affecting the genetic structure of L.brandtii populations.
作者
刘雨秋
鲁亮
刘蓬勃
赵宁
李贵昌
栗冬梅
宋秀平
王君
刘起勇
LIU Yu-qiu;LU Liang;LIU Peng-bo;ZHAO Ning;LI Gui-chang;LI Dong-mei;SONG Xiu-ping;WANG Jun;LIU Qi-yong(State Key Laboratory of Infectious Disease Prevention and Control,Department of Vector Biology and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)
出处
《中国媒介生物学及控制杂志》
CAS
北大核心
2023年第3期291-297,共7页
Chinese Journal of Vector Biology and Control
基金
国家自然科学基金重大项目(32090023)。
