摘要
为进一步解析镉胁迫对不同镉耐受性香菇菌株生长发育的影响,明确镉胁迫下香菇子实体不同发育阶段生理变化规律,以不同镉耐受性香菇菌株为材料,通过外源添加镉的方式探究两个菌株不同发育阶段子实体中镉含量、丙二醛(MDA)含量、可溶性蛋白含量的变化规律。结果表明:镉胁迫对两个菌株子实体农艺性状和产量都产生影响,但不同镉耐受性菌株受影响的农艺性状和受影响程度不同。菌株4606受到镉胁迫后的菌盖直径、菌盖厚度显著降低,其余农艺性状未受到显著影响;菌株4625受到镉胁迫后的单棒子实体个数、单棒产量显著降低,单菇质量、菌盖质量极显著升高,其余农艺性状未受到显著影响。两个菌株不同生长发育阶段单位干质量子实体中镉含量均有随着生长发育时间的延长而显著降低的趋势。受到镉胁迫后菌株4606的MDA含量在生长期显著升高,成熟期显著下降,开伞期极显著降低;菌株4625受到镉胁迫后开伞期的MDA含量显著降低,其余阶段未受到显著影响。菌株4606对照组与镉胁迫处理组的可溶性蛋白含量均随生长时间延长显著降低,菌株4625对照组与镉胁迫处理组的可溶性蛋白含量均随生长时间延长先显著升高后显著降低;镉胁迫导致菌株4606开伞期的可溶性蛋白含量显著降低,而菌株4625在原基期、生长期、开伞期的蛋白含量均极显著升高。综上,不同镉耐受性香菇菌株子实体对镉胁迫的生理响应存在一定差异,因此,在香菇生产上应选取低镉吸收品种进行农业生产。
In this study,we analyzed the cadmium(Cd)accumulation characteristics of different Cd-tolerant Lentinula edodes strains and the physiological changes in Lentinula edodes fruiting bodies at different developmental stages under Cd stress.As exogenous Cd stress was added,and Cd content changed,malondialdehyde(MDA)and soluble protein content in fruiting bodies at different developmental stages were explored.The results show that Cd stress affected the agronomic characteristics and yield of the fruiting bodies of the two strains,but different Cd tolerant strains were affected differently.The diameter and thickness of the cap of strain 4606 decreased significantly after Cd stress,but the other agronomic traits showed no significant difference.After Cd exposure to strain 4625,the number of fruiting bodies and the yield per rod decreased significantly,and the weight of a single mushroom and the weight of cap increased significantly.The Cd content in the fruiting bodies of unit dry mass of the two strains decreased significantly with the extension of growth and development time.After Cd stress,the MDA content of strain 4606 increased significantly in the growth period,decreased significantly in the mature period,and decreased extremely significantly in the parachute stage.The protein content of the 4606 strain control group and the Cd stress treatment group decreased significantly with a longer growth time,and that of the 4625 strain control group and the Cd stress treatment group increased significantly at first and then decreased significantly over time.The protein content of the 4606 strain at the open capillary stage was significantly reduced,but there were no significant difference in the other stages.In conclusion,the physiological responses of fruiting bodies to Cd stress of different Cd-tolerant strains of Lentinula edodes were different.Therefore,varieties with low Cd absorption should be selected for agricultural production.
作者
翟丹丹
闫苗苗
姜宁
李巧珍
尚晓冬
谭琦
李玉
于海龙
ZHAI Dandan;YAN Miaomiao;JIANG Ning;LI Qiaozhen;SHANG Xiaodong;TAN Qi;LI Yu;YU Hailong(Engineering Research Centre of Chinese Ministry of Education for Edible and Medicinal Fungi,Jilin Agricultural University,Changchun 130118,China;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Key Laboratory of Edible Fungal Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,P.R.China,National Engineering Research Center of Edible Fungi,National R&D Center for Edible Fungal Processing,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403,China)
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2023年第6期1267-1273,共7页
Journal of Agro-Environment Science
基金
山东省重点研发计划项目(20221ZGC023)
国家食用菌产业技术体系项目(CARS-20)
山东省泰山产业领军人才项目(LJNY202116)。
关键词
镉胁迫
香菇
生长发育
农艺性状
生理指标
Cd stress
Lentinula edodes
growth and development
agronomic traits
physiological indicators
