摘要
目的探究重度子痫前期(PE)患者外周血miR⁃221、miR⁃651⁃3p及杀伤细胞抑制性受体(KIR)表达水平及临床意义。方法回顾性分析2019年3月—2022年8月于合肥市第一人民医院南区就诊143例PE患者临床资料。根据PE严重程度将患者分为重度PE组(n=85)和轻度PE组(n=58),另选取同期正常妊娠孕妇65例为对照组。比较3组及PE患者中不同妊娠结局患者的外周血miR⁃221、miR⁃651⁃3p、KIR表达水平;受试者工作特征曲线(ROC)评估miR⁃221、miR⁃651⁃3p、KIR对重度PE的诊断效能;Logistic回归分析miR⁃221、miR⁃651⁃3p、KIR与PE患者妊娠结局的关系。结果与对照组比较,轻度PE组、重度PE组外周血miR⁃221、miR⁃651⁃3p、KIR表达水平升高,且重度PE组高于轻度PE组(P<0.05)。ROC曲线分析结果显示,miR⁃221、miR⁃651⁃3p、KIR诊断重度PE的AUC值分别为0.836、0.835和0.879(P<0.05),最佳阈值分别为1.43、1.70和52.13μg/L。各组不良妊娠结局发生率比较差异有统计学意义(P<0.05),且重度PE组最高,轻度PE组高于对照组(P<0.05)。轻度及重度PE患者中,结局不良患者外周血miR⁃221、miR⁃651⁃3p、KIR水平均高于结局良好者(P<0.05)。Logistic回归分析结果显示,重度PE、miR⁃221、miR⁃651⁃3p、KIR与PE患者不良妊娠结局呈正相关(P<0.05)。结论PE患者外周血miR⁃221、miR⁃651⁃3p、KIR表达水平偏高,并与病情严重程度相关,可能会增加PE患者不良妊娠结局风险。检测外周血miR⁃221、miR⁃651⁃3p、KIR水平或可为临床重度PE的诊断提供参考。
Objective To investigate the expression levels and clinical significance of miR⁃221,miR⁃651⁃3p and killer⁃cell inhibitory receptor(KIR)in peripheral blood of patients with severe preeclampsia(PE).Methods The clinical data of 143 patients with PE who were treated in Hefei First People's Hospital from March 2019 to August 2022 were retrospectively analyzed.According to the severity of PE,the patients were divided into severe PE group(n=85)and mild PE group(n=58).Meanwhile,65 normal preg⁃nant women were selected as the control group.Peripheral blood miR⁃221,miR⁃651⁃3p and KIR expression levels in the three groups and in PE patients with different pregnancy outcomes were comparatively analyzed.The diagnostic performance of miR⁃221,miR⁃651⁃3p and KIR for severe PE was evaluated using the receiver operating characteristic(ROC)curve.Logistic regression analysis was performed to analyze the relationship between miR⁃221,miR⁃651⁃3p,KIR and pregnancy outcomes of PE patients.Results Compared to the control group,the expression levels of miR 221,miR 6513p,and KIR in the peripheral blood of the mild PE group and the severe PE group were increased,and the level of the severe PE group was higher than that of the mild PE group(P<0.05).ROC curve analysis showed that the AUC values of miR⁃221,miR⁃651⁃3p and KIR for severe PE patients were 0.836,0.835 and 0.879,respectively(P<0.05).The optimal thresholds were 1.43μg/L,1.70μg/Land 52.13μg/L,respectively.The incidence of adverse pregnancy outcomes among the groups is statistically significant(P<0.05),and the severe PE group was the highest,and the mild PE group was higher than the control group(P<0.05).In the mild PE group and the severe PE group,peripheral bloodmiR⁃221,miR⁃651⁃3p and KIR levels in patients with poor outcomes were higher than those in patients with good outcomes(P<0.05).Logistic regression analysis found that severe PE,miR⁃221,miR⁃651⁃3p and KIR were posi⁃tively correlated with adverse pregnancy outcomes of PE patients(P<0.05).Conclusion Peripheral blood miR⁃221,miR⁃651⁃3p and KIR expression levels in patients with PE are relatively high and are related to the severity of PE.The elevated expression levels may increase the risk of adverse pregnancy outcomes in patients with PE.Monitoring the levels of miR⁃221,miR⁃651⁃3p and KIR in peripheral blood may provide reference for diagnosing severe PE.
作者
刘荃
韦芳琴
LIU Quan;WEI Fangqin(Department of Obstetrics and Gynecology,Hefei First People′s Hospital,Hefei 230001,Anhui,China)
出处
《医学研究与战创伤救治》
CAS
北大核心
2023年第3期274-279,共6页
Journal of Medical Research & Combat Trauma Care
