摘要
目的:比较去势结合雌激素诱导法与自身免疫反应诱导法建立慢性非细菌性前列腺炎(CNP)模型大鼠的生物学特性及稳定性。方法:去势激素组采用去势结合17β-雌二醇诱导法、自身免疫组分别采用40、20、10 mg/mL三种浓度前列腺蛋白匀浆制备CNP大鼠模型,采用病理学(HE染色)评价前列腺组织损伤程度,免疫比浊法测定免疫球蛋白IgA、IgM、IgG含量,ELISA法测定白细胞介素-1β(IL-1β)、白细胞介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)、高敏-C反应蛋白(hs-CRP),电化学发光法测定睾酮(T)表达水平,应用上诉指标对两种模型方法进行比较。结果:与假手术组比较,取材前去势激素组和自身免疫组两种模型大鼠体质量均显著降低(P<0.01,P<0.01),两种模型大鼠前列腺组织病理显示腺体结构破坏,炎性细胞明显增多。去势激素组同自身免疫组比较,去势激素组前列腺组织病理变化和炎症病理评分、免疫指标IgA、IgM、IgG含量均具有显著差异(P<0.05,P<0.05,P<0.05),自身免疫组IL-1β、IL-10、TNF-α、hs-CRP、T水平显著改变(P<0.01)。自身免疫组高、中、低剂量组三者之间比较,其中自身免疫组中剂量病理变化稍优于组间其它两组,但自身免疫组低剂量IL-1β、IL-10、TNF-α、hs-CRP、T改变最为显著(P<0.05)。结论:去势结合雌激素诱导法和自身免疫反应诱导法建立的慢性非细菌性前列腺炎模型的病理组织均显示显著变化,综合指标显示去势结合雌激素诱导法是更为合适的造模选择。
Objective:To compare the biological characteristics and stability of the chronic non-bacterial prostatitis(CNP)model in rats induced by the depot combined with estrogen induction method and the autoimmune response induction method.Methods:The CNP rat model was prepared using the depot combined with 17β-estradiol induction method in the depot hormone group and three concentrations of prostate protein homogenate at 40 mg/mL,20 mg/mL and 10 mg/mL in the autoimmune group,respectively.The degree of prostate tissue damage was evaluated by pathology(HE staining),and the immunoturbidimetric method was used to evaluate the contents of immunoglobulins IgA,IgM and IgG.ELISA was used to evaluate interleukin-1β(IL-1β),interleukin-10(IL-10),tumor necrosis factor-α(TNF-α)and high-sensitivity-C-reactive protein(hs-CRP),and electrochemiluminescence was used to evaluate the expression level of testosterone(T),and the two model methods were compared.Results:Compared with the sham-operated group,the body mass of rats in both models was significantly lower in the depot hormone group and autoimmune group before extraction(P<0.01,P<0.01),and histopathology of the prostate in both models showed destruction of glandular structure and a significant increase in inflammatory cells.Compared with the depot hormone group and the autoim mune group,the histopathological changes and inflammatory pathological scores of prostate and the contents of immune indexes IgA,IgM and IgG were significantly different in the depot hormone group(P<0.05,P<0.05,P<0.05),and the levels of IL-1β,IL-10,TNF-α,hs-CRP and T were significantly changed in the autoimmune group(P<0.01).The comparison between the high,medium and low dose groups of the autoimmune group,in which the pathological changes in the medium dose of the autoimmune group were slightly better than the other two groups between the groups,but the changes in IL-1β,IL-10,TNF-α,hs-CRP,and T in the low dose of the autoimmune group were the most significant(P<0.05).Conclusion:The pathological tissues of the chronic non-bacterial prostatitis model established by the depot combined with estrogen induction method and the autoimmune response induction method both showed significant changes,and the comprehensive indexes indicated that the depot com bined with estrogen induction method was a more appropriate modeling choice.
作者
唐益文
王雄
周艳艳
陈豪特
张泽家
王忠
高庆和
刘剑刚
高瞻
TANG Yi-wen;WANG Xiong;ZHOU Yan-yan;CHEN Hao-te;ZHANG Ze-jia;WANG Zhong;GAO Qing-he;LIU Jian-gang;GAO Zhan(Graduate School of Beijing University of Chinese Medicine,Beijing 100029,China;Department of Urology,Xiyuan Hospital,Chinese Academy of Traditional Chinese Medicine,Beijing 100091,China;Department of Male Medicine,Xiyuan Hospital,Chinese Academy of Traditional Chinese Medicine,Beijing 100091,China;Institute of Cardiovascular Diseases,Chinese Academy of Traditional Chinese Medicine,Beijing 100091,China)
出处
《海南医学院学报》
2023年第13期961-967,共7页
Journal of Hainan Medical University
基金
国家自然科学基金项目(82104881)
中国中医科学院科技创新工程重大攻关项目(CI2021A02205)。
