摘要
目的前噬菌体是细菌基因组中重要可移动原件,在细菌毒力和宿主适应性方面发挥着重要作用。由噬菌体介导的福氏志贺菌血清型转化频繁发生,给志贺菌的监测、诊断和预防工作带来不便。为了探究我国福氏志贺菌基因组进化与其前噬菌体相关的血清型别转换之间的关系,利用生物信息学方法将前噬菌体在福氏志贺菌基因组中的分布与进化进行关联分析。方法在中国17省(市、自治区)选取的294株福氏志贺菌具有丰富的血清型多态性。通过比较不同血清型福氏志贺菌基因组中原噬菌体及血清型转换噬菌体的覆盖度,统计分析其分布规律。结果部分菌株可携带多种前噬菌体,同一种前噬菌体也可分布在不同血清型菌株中;部分菌株虽携带血清型转换噬菌体却并未发生血清型转换事件。不同血清型转换噬菌体在福氏志贺菌进化树中成簇出现,表明该部分菌株出现了血清型集中转换现象。结论本研究有助于揭示前噬菌体的遗传多样性及与福氏志贺菌进化和血清型变异之间的关系,对预防和控制志贺菌的感染具有重要意义。
Objective Prophage is one of the mobile genetic elements carried on bacterial genome,which plays an important role in bacterial virulence and host adaptability.Phage-mediated serotype conversion occurs frequently,which brings inconvenience to the surveillance,diagnosis and prevention of Shigella.In order to explore the relationship between the serotype-converting phages of Shigella flexneri and genome evolution,we analyzed correlation between the distribution of prophage and the phylogenetic tree of Shigella flexneri.Methods In total of 294 Shigella flexneri with different serotypes from 17 provinces in China were selected.We compared the coverage of prophages and serotype-converting phages in the different genome of Shigella flexneri.Correlation analysis were did about the phage distribution in phylogenetic tree.Results The results showed that multiple kinds of prophages were carried by Shigella flexneri,and the same prophage may distributed in different serotype strains.Some strains carried bacteriophages without serotype-converting events.The bacteriophages appeared in clusters in the evolutionary tree,indicating a concentrated serotype-converting events.Conclusion We indicated the close relationship between serotype-converting phages and the evolution of Shigella flexneri.Our research is of great significant to prevent and control Shigella infection.
作者
梁俊容
金东
杨晶
卢珊
熊衍文
叶长芸
徐建国
Liang Junrong;Jin Dong;Yang Jing;Lu Shan;Xiong Yanwen;Ye Changyun;Xu Jianguo(Institute for Infectious Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)
出处
《疾病监测》
CAS
CSCD
北大核心
2023年第5期516-521,共6页
Disease Surveillance
基金
国家重点研发计划项目(No.2021YFC0863400,No.2021YFC2302000)
未知细菌发现与功能研究(No.2018RU010)。
关键词
福氏志贺菌
噬菌体
血清型转换
细菌基因组
进化
Shigella flexneri
Prophage
Serotype conversion
Bacterial genome
Evolution
