摘要
SPL转录因子广泛参与植物生长发育、胁迫响应等过程。目前,没有关于铁皮石斛SPL膜结合(SPL with transmembrane motif)转录因子即STM转录因子的研究。为了探究STM转录因子在铁皮石斛生长发育及胁迫响应等方面的作用,该文在铁皮石斛全基因组水平鉴定出4个STM转录因子,并对DoSTM基因家族成员进行生物信息学分析,又利用逆转录PCR研究了DoSTM在不同组织部位及不同逆境处理下的表达情况。结果表明:(1)DoSTM1-4为亲水蛋白,均具有SBP保守结构域和一些激素响应位点。(2)4个DoSTM在根茎叶中均有表达,DoSTM 2在叶中的相对表达量最低;DoSTM 1/3/4的相对表达水平均无明显差异。(3)DoSTM 1-4在低温、高温、干旱胁迫下的相对表达水平都有显著变化,DoSTM 1/3/4的表达量降低最为明显,故推测DoSTM与植物体内激素响应、温度变化响应及抗旱性有关。这些结论为后续进一步开展铁皮石斛STM转录因子的研究提供了参考。
SPL transcription factors are widely involved in plant growth and development,stress response and so on.At present,there is no study on the STM(SPL with transmembrane motif)transcription factor in Dendrobium officinale.In order to explore the role of STM in the growth,development and stress response of D.officinale,four STM transcription factors were identified at the whole genome of D.officinale,and bioinformatics analysis of DoSTM gene family members were carried out.The expression of DoSTM in different tissue parts and different stress treatments were studied by reverse transcription PCR.The results were as follows:(1)DoSTM1-4 were hydrophilic proteins with SBP conserved domains and some hormone response sites.(2)Four DoSTM were expressed in root,stem and leaf,and the relative expression of DoSTM 2 was the lowest in leaf;there was no significant differences in the relative expression level of DoSTM 1/3/4.(3)The relative expression level of DoSTM 1-4 changed significantly under low temperature,high temperature and drought stress,and the expression of DoSTM 1/3/4 decreased most significantly.Therefore,it is speculated that DoSTM is related to hormone response,temperature change response and drought resistance in plants.These conclusions provide the reference for the further research on STM transcription factor of D.officinale.
作者
杨乐
聂聪
龙小琴
何基泽
颜超越
朱乾坤
王万军
YANG Le;NIE Cong;LONG Xiaoqin;HE Jize;YAN Chaoyue;ZHU Qiankun;WANG Wanjun(School of Life Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China)
出处
《广西植物》
CAS
CSCD
北大核心
2023年第6期1041-1050,共10页
Guihaia
基金
国家自然科学基金(31900164)
中央高校基本科研业务费(2682021CX121)
西南交大个性化实验项目(GX2021160027)。
关键词
铁皮石斛
STM转录因子
基因鉴定
功能分析
基因表达
Dendrobium officinale
STM transcription factor
gene identification
functional analysis
gene expression
