摘要
川崎病(KD)是一种主要累及全身中小血管的急性发热性疾病,好发于5岁以下儿童,冠状动脉病变是其主要并发症。目前KD已成为发达国家儿童获得性心脏病最常见的原因。不同于传统测序方法,单细胞测序技术在单细胞分辨率下对遗传信息进行测序,不仅能很好地解决细胞异质性问题,还能鉴定细胞亚群、寻找生物标志物、绘制细胞谱系。文章介绍了单细胞测序技术的发展及其在KD研究领域的应用现状,并对其发展前景进行展望。
Kawasaki disease(KD)is an acute febrile illness which mainly involves the small and medium-sized blood vessels throughout the body,predominantly occurs in children under 5 years old.Coronary artery lesions are its main complication and KD is now the commonest cause of acquired heart disease in children in developed countries.Different from traditional sequencing methods,single-cell sequencing technology can sequence genetic information at single-cell resolution,which can not only solve the problem of cell heterogeneity,but also identify cell subsets,search for biomarkers,and map cell lineages.This commentary will describe the development of single cell sequencing technology and its current application in the field of KD,and provide an outlook on its development.
作者
黄敏
HUANG Min(Department of Cardiology,Shanghai Children’s Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai 200062,China)
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2023年第7期481-485,共5页
Journal of Clinical Pediatrics
基金
国家自然科学基金项目(No.82170518)
上海市科委“科技创新行动计划”医学创新研究专项项目(No.21Y31900304)
上海市科委“科技创新行动计划”技术标准项目(No.21DZ2201400)
上海申康医院发展中心2021年临床科技创新项目(No.SHDC22021305)。
关键词
单细胞测序技术
川崎病
细胞谱系
single cell sequencing
Kawasaki disease
cell lineage
