摘要
芥菜(Brassica juncea)对多种重金属具有富集能力。重金属ATP酶(heavy metal transporting ATPase,HMA)在植物转运重金属过程中具有重要的作用。基于基因组和转录组数据,对芥菜HMA家族基因成员进行了全基因组鉴定。芥菜基因组中包含27个HMA基因,其编码的蛋白质中有6个不稳定指数大于40,有22个为疏水性蛋白;这些基因聚类为P-1B-1、P-1B-2和P-1B-4等3个亚家族;27个HMA蛋白均为膜蛋白,都具有E1-E2ATPase和hydrolase结构域;芥菜HMA蛋白都含有8~15个保守基序,不同亚族因具有独特的保守基序结构从而转运重金属的种类不同;在芥菜HMA基因启动子区域中与生长发育有关的顺式作用元件TGA和与逆境响应相关的顺式作用元件ABRE、MBS、LTR、TC广泛分布;在镉胁迫下,BjuA033764、BjuB019118、BjuB035256、BjuB045293等基因在叶片中表达量明显上调,BjuA003596、BjuB040613、BjuA025022等基因在根系中表达量明显上调,说明其参与了芥菜对镉胁迫的响应。
Brassica juncea can enrich a variety of heavy metals.Heavy metal transporting ATPase(HMA)plays an important role in the process of transporting heavy metals in plants.Based on genomic and transcriptomic data,the HMA gene family members in B.juncea were identified.A total of 27 HMA genes were found in B.juncea genome.Six of these HMA proteins have instability indexes greater than 40.Twenty-two members are hydrophobic proteins.These genes cluster into three subfamilies,P-1B-1,P-1B-2,and P-1B-4.All the HMAs are membrane proteins containing the E1-E2 ATPase and hydrolase domains.Each HMA protein contains 1-8 motifs.Different subfamilies have unique conserved motifs according to the types of heavy metals transported by them.In the promoter regions of the HMA genes,the cis-acting element TGA related to the regulation of growth and development,the cis-acting elements ABRE,MBS,LTR,and TC related to the regulation of stress response are widely distributed.Under cadmium(Cd)stress,BjuA033764,BjuB019118,BjuB035256,and BjuB045293 were up-regulated in leaves,and BjuA003596,BjuB040613,and BjuA025022 were up-regulated in roots,indicating their involvement in the response to Cd stress.
作者
黄治皓
刘婷婷
董旭杰
严明理
刘志祥
曾超珍
HUANG Zhihao;LIU Tingting;DONG Xujie;YAN Mingli;LIUZhixiang;ZENG Chaozhen(Hunan Provincial Key Laboratory of Forestry Biotechnology,College of Life Science and Technology,Central South University of Forestry and Technology,Changsha 410004,China;Crop Research Institute,Hunan Academy of Agricultural Sciences,Changsha 410125,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2023年第6期1230-1242,共13页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31671601)
湖南省自然科学基金项目(2021JJ31139)
湖南省教育厅科学研究重点项目(21A0148)
湖南省研究生科研创新项目(CX20210896)。
关键词
芥菜
重金属ATP酶
HMA基因家族
镉胁迫
结构域
顺式作用元件
Brassica juncea
heavy metal transporting ATPase
HMA gene family
cadmium stress
domain
cis-acting element
