摘要
探究GA3、ABA和6-BA三种植物激素对冬瓜果实品质的影响并进一步探究其作用机制。以冬瓜(B481)为试材,测得外源喷施激素后冬瓜果实外观、风味、营养品质的变化及相关基因表达的变化。ABA处理后冬瓜果长及果粗显著减少,且与冬瓜果型相关的生长素响应基因BhSAUR的表达显著下调;GA3处理后冬瓜果实果糖和葡萄糖含量显著提高、蔗糖含量显著降低,且糖代谢通路中碱性α-半乳糖苷酶BhAGA1与BhAGA2、酸性转化酶BhSAI1及蔗糖合成酶BhSUS2的表达显著上调、蔗糖合成酶BhSUS4及蔗糖磷酸合成酶BhSPS1的表达显著下调;GA3、ABA、6-BA分别处理后冬瓜果实中AsA含量均显著降低,且冬瓜抗坏血酸代谢通路中脱氢抗坏血酸还原酶BhDHAR2的表达显著上调。综上,植物激素通过调控关键基因的表达影响冬瓜果长、单糖、蔗糖、抗坏血酸。这为激素改善冬瓜果实品质提供重要的理论依据。
The study aims to explore the effects of three phytohormones GA3,ABA and 6-BA on fruit quality of wax gourd and further explore their mechanism of action.Taking wax gourd(B481)as the test material,the changes of fruit appearance,flavor and nutritional quality,and the expression of related genes after treatments of the three phytohormones were measured.The fruit length and diameter of wax gourd treated with ABA were significantly decreased,and the expression of auxin-responsive gene BhSAUR related to fruit type was significantly down-regulated.The content of fructose and glucose increased significantly and the content of sucrose decreased significantly in wax gourd fruits under GA3 treatment.The expression of alkalineα-galactosidase BhAGA1 and BhAGA2,acid invertase BhSAI1 and sucrose synthase BhSUS2 in sugar metabolic pathway were significantly up-regulated,while the expression of sucrose synthase BhSUS4 and sucrose phosphate synthase BhSPS1 were significantly down-regulated.The content of AsA in wax gourd fruits treated with GA3,ABA and 6-BA decreased significantly,and the expression of dehydroascorbate reductase BhDHAR2 in ascorbic acid metabolism pathway was significantly up-regulated.In summary,phytohormones affect wax gourd fruit length,monosaccharide content,sucrose content and ascorbic acid content by regulating the expression of key genes.The study can provide a theoretical basis for using phytohormones to improve the fruit quality of wax gourd.
作者
薛舒丹
万小童
钟玉娟
吴玉娟
陆森
刘展舒
傅曼琴
谢大森
XUE Shudan;WAN Xiaotong;ZHONG Yujuan;WU Yujuan;LU Sen;LIU Zhanshu;FU Manqin;XIE Dasen(Vegetable Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Vegetable Technology Research,Guangzhou 510640;Huazhong Agricultural University,Wuhan 318020;Sericultural&Agri-Food Research Institute,Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods,Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing,Guangzhou 510610)
出处
《中国农学通报》
2023年第18期52-60,共9页
Chinese Agricultural Science Bulletin
基金
广东省重点领域研发计划资助项目“优质加工型蔬菜新品种选育”(2020B020220003),“优质多抗岭南特色瓜类蔬菜新品种选育”(2018B020202007),“广东地方蔬菜品种提纯复壮与创新利用”(2020B0202090002)
广州市科技计划项目“冬瓜(精-瓜)氨酸积累关键酶基因挖掘及功能初探”(202201010231)
岭南现代农业实验室项目“岭南特色果蔬抗病机制、种质创新与新品种选育研究”(NT2021004)
广东省农业科学院院创新基金青年自然科学基金面上项目“基于多组学的冬瓜减脂物质丙醇二酸合成基因的挖掘研究”(202106)
广东省农业科学院协同创新中心课题项目“岭南特色果蔬多维品质数据库构建与应用及精深加工研究”(XT202226)
广东省蔬菜新技术重点实验室开放课题基金项目“冬瓜功能营养成分丙醇二酸QTL定位”(201905)
广东省现代农业产业技术体系项目“果蔬加工共性关键技术研究”(2022KJ117)。
关键词
外源激素
冬瓜
外观品质
风味品质
营养品质
相关基因表达
exogenous phytohormones
wax gourd
appearance quality
flavor quality
nutritional quality
related gene expression
