摘要
[目的]微生物VOCs可作为信号分子,在不同物种间的相互作用中起重要作用。它可以直接或间接地调节植物生长发育和耐逆性。本文旨在初步探明黄绿卷毛菇(Floccularia luteovirens)挥发性有机化合物(volatile organic compounds,VOCs)对白菜型油菜(Brassica rapa)生长影响的机理。[方法]本研究通过生理学与转录组学方法,研究了黄绿卷毛菇VOCs对白菜型油菜幼苗生长的影响。测量了黄绿卷毛菇VOCs处理12 d后白菜型油菜的生物量、可溶性糖含量及光合指标,提取了黄绿卷毛菇VOCs处理了2 d后油菜叶片的总RNA进行了转录组测序、RT-qPCR验证,并对转录组数据进行了GSEA、GO及KEGG分析。[结果]黄绿卷毛菇VOCs处理增加了油菜幼苗生物量,提高了叶绿素含量、光合指标和叶片可溶性糖含量。转录组学分析结果表明,黄绿卷毛菇VOCs处理后,叶片中有338个差异表达基因(DEGs),其中220个DEGs表达上调,118个DEGs表达下调。GSEA分析结果表明,有丝分裂细胞周期、肌球蛋白结合、跨膜转运蛋白活性、激素介导的信号通路、光系统Ⅱ等途径被诱导。GO富集分析结果表明,大部分GO条目与蛋白折叠、与氧化还原酶活性和与含硫S化合物相关的跨膜转运蛋白有关。KEGG富集分析结果表明,DEGs主要富集在植物-病原体相互作用、淀粉和蔗糖代谢、碳代谢、苯丙烷类生物合成、RNA降解、氨基糖和核苷酸糖代谢等途径。[结论]黄绿卷毛菇VOCs通过调节光合作用、营养平衡、激素途径和代谢途径,影响白菜型油菜生长,并且可能在植物响应逆境胁迫中发挥作用。
[Objective]Microbial volatile organic compounds(VOCs)can serve as signaling molecule and play important roles in the interactions between different species.Microbial VOCs can directly or indirectly regulate plant growth,development,and stress tolerance.The purpose of this paper was to preliminarily explore the mechanism of VOCs from Floccularia luteovi⁃rens on the growth of Brassica rapa seedlings.[Methods]In this study,physiological and transcriptomic approaches were used to investigate the effects of VOCs from F.luteovirens on the growth of B.rapa seedlings.The biomass,soluble sugar con-tent,and photosynthetic indexes of B.rapa seedlings were measured after treatment with VOCs from F.luteovirens for 12 days.Total RNA was extracted from B.rapa leaves after two days of VOCs treatment and subjected to transcriptome sequenc-ing and RT-qPCR verification.Transcriptome data were analyzed by gene set enrichment analysis(GSEA),gene ontology(GO),and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis.[Results]Treatment with VOCs increased the bio-mass of seedlings and enhanced chlorophyll content,photosynthetic indexes,and leaf soluble sugar content in B.rapa seed-lings.Transcriptomic analysis revealed 338 differentially expressed genes(DEGs)in the leaves after VOCs treatment,includ-ing 220 upregulated DEGs and 118 downregulated DEGs.GSEA analysis showed the induction of pathways related to mitotic cell cycle processes,myosin binding,transmembrane transporter activity,hormone-mediated signaling,and photosystem II.GO enrichment analysis indicated that most GO terms were associated with protein folding,oxidoreductase activity,and trans-membrane transporters related to sulfur-containing compounds.KEGG enrichment analysis showed that DEGs were mainly en-riched in pathways such as plant–pathogen interactions,starch and sucrose metabolism,carbon metabolism,phenylpropanoid biosynthesis,RNA degradation,and amino sugar and nucleotide sugar metabolism.[Conclusion]These results suggested that VOCs from F.luteovirens positively affect the plant growth by regulating photosynthesis,nutrient balance,hormonal path-ways,and metabolic pathways.Furthermore,these VOCs from F.luteovirens may play a role in plant response to stress and adversity.
作者
丁肖月
孙亮亮
刘飞
张苹
李卫敏
徐进
Ding Xiaoyue;Sun Liangliang;Liu Fei;Zhang Ping;Li Weimin;Xu Jin(College of Horticulture,Shanxi Agricultural University,Jinzhong 030801,China)
出处
《山西农业大学学报（自然科学版）》
CAS
北大核心
2023年第3期33-41,共9页
Journal of Shanxi Agricultural University(Natural Science Edition)
基金
山西农业大学科技创新基金项目(2020QC13)
山西省研究生创新项目(2021Y307)。
关键词
挥发性有机化合物
黄绿卷毛菇
白菜型油菜
生长
光合效率
基因表达
Volatile organic compound
Floccularia luteoviren
Brassica rapa
Growth
Photosynthetic efficiency
Gene ex-pression
