摘要
为研究Osp39基因在水稻叶绿体蛋白质输入调控过程中的作用,采用生物信息学方法对水稻的Osp39基因进行了序列搜索和启动子上游元件分析,对其编码的OsP39蛋白进行了同源性比对、结构和性质预测,并分别通过定量PCR和瞬时表达进行了基因的组织表达分析和蛋白质亚细胞定位分析。结果显示,Osp39基因位于水稻5号染色体,基因编码区含11个外显子,无可变剪切方式。Osp39启动子中含有I box、ATCT-motif等多种光响应和叶绿体调节元件,转录分析也显示,在苗期和扬花期,Osp39基因在水稻叶、叶鞘等绿色组织中的转录水平均极显著高于根、花等非绿色组织。OsP39蛋白稳定性好,耐热,分子量约38.7 ku,理论等电点pH值8.64,共含有361个氨基酸残基,富含甘氨酸,第245—271氨基酸残基形成高度保守的L6 loop区,为OMP85蛋白家族的特征性结构域,空间结构预测显示,OsP39蛋白是富含β-折叠片的β-桶状膜蛋白,亚细胞定位结果显示,OsP39蛋白定位于水稻叶绿体膜。上述结果表明,Osp39是一个叶绿体膜功能相关基因,参与水稻的光响应和叶绿体功能调节。
To explore the role of Osp39 gene in the regulation of rice chloroplast protein import,the sequence of Osp39 gene and upstream elements in its promoter were searched and analyzed by using bioinformatics assay,and the homology,properties and structure of the OsP39 protein were also carried out.The tissue expression of Osp39 gene and subcellular localization of OsP39 protein were analyzed by quantitative PCR and transient expression in protoplasts,respectively.The results showed that the Osp39 gene was located on chromosome 5 of rice.There were 11 exons in the coding region of Osp39 gene,and its transcription product had no alternative splicing mode,so there was only one type of transcription product.The Osp39 promoter contained a variety of light-responsive and chloroplast regulatory elements such as I box and ATCT-motif.Transcriptional analysis of Osp39 gene also showed that the transcription level of Osp39 gene in green tissues such as leaf and leaf sheath was significantly than that in non-green tissue such as root and flower during seeding and flowering stages.OsP39 protein was stable and heat-resistant,which molecular weight was about 38.7 ku and theoretical isoelectric point was pH 8.64.There were a total of 361 amino acid residues in OsP39 protein,of which the content of glycine was the highest.The 245-271 amino acid residues formed a highly conserved L6 loop region,which belonged to the characteristic domain of the OMP85 protein family.Spatial structural analysis revealed that OsP39 was aβ-barrel membrane protein rich inβ-sheets.Subcellular localization analysis showed that OsP39 protein was localized at the rice chloroplast membrane.The above results indicated that Osp39 gene was a chloroplast membrane related gene,which was involved in the regulation of light response and chloroplast function in rice.
作者
腾海艳
TENG Haiyan(School of Chemistry and Bioengineering,Yichun University,Yichun 336000,China)
出处
《华北农学报》
CSCD
北大核心
2023年第3期10-17,共8页
Acta Agriculturae Boreali-Sinica
基金
江西省教育厅科学技术研究项目(GJJ211619)。
