摘要
建立高效液相色谱法测定甘蔗渣盐酸催化水解液中的糖组分。采用Aminex HPX-87P色谱柱(300mm×7.8 mm,粒径9μm,交联率8%),以超纯水为流动相,流速为0.60 mL/min,柱温75℃,进样量10μL。以峰面积定量,结果表明:D-葡萄糖、D-木糖、D-半乳糖、L-阿拉伯糖分别在0.5000~5.000 mg/mL(R=0.9996),2.000~20.00 mg/mL (R=0.9999),0.0260~0.2600 mg/mL (R=0.9991),0.2000~2.000 mg/mL(R=0.9996)范围内有良好的线性关系,平均回收率(n=9)分别为99.05%、99.53%,98.50%、98.63%,重复性实验中,4种糖含量的RSD(n=5)分别为0.13%、0.05%、0.86%、0.46%。本试验方法效率高、重现性及回收率好,可有效应用于甘蔗渣盐酸水解液糖组分的检测。
HPLC was established to determine the sugar components in the acid hydrochloride catalyzed hydrolysate of bagasse.Methods Aminex HPX-87P column(300 mm×7.8 mm,particle size 9μm,8%)was adopted,ultrapure water was used as mobile phase at a flow rate of 0.60 mL/min,the column temperature was set at 80℃,and the injection volume was 10μL.By the peak area,the results showed that the D-Glucose,D-Xylose,D-Galactose,and L-Arabinose had good linearity in the range of 0.2000~2.000 mg/mL(R=0.9996),2.000~20.00 mg/mL(R=0.9999),0.0260~0.2600 mg/mL(R=0.9991),0.2000~2.000 mg/mL(R=0.9996),respectively;The average recovery rate(n=9)were 99.05%,99.53%,98.50%,98.63%,respectively.In the repeatability experiments,the RSD(n=5)for the four sugar contents were 0.13%,0.05%,0.86%,and 0.46%,respectively.The method had high efficiency,good repeatability and good recovery rate,and can be effectively used for sugarcane bagasse hydrochloric acid hydrolysate detection.
作者
牙冬梅
王元春
张荣立
黄志钰
冯德伟
易立鹏
谢承孟
YA Dongmei;WANG Yuanchun;ZHANG Rongli;HUANG Zhiyu;FENG Dewei;YI Lipeng;XIE Chengmeng(Guangxi Light Industry Science and Technology Research Institute Co.Ltd.,Nanning,Guangxi 530031)
出处
《甘蔗糖业》
2023年第3期48-54,共7页
Sugarcane and Canesugar
基金
广西重点研发计划项目(桂科AB20159045)。
