摘要
从湖北省恩施土家族苗族自治州富硒土壤中筛选出一株高耐硒菌株并进行菌种鉴定,检测了该菌株对亚硒酸钠的还原能力,表征了其经生物转化生成纳米硒的生物学特性,并对该菌株产吲哚乙酸(Indoleacetic acid,IAA)的能力进行了测定。16S rDNA序列分析和生理生化鉴定结果表明该高耐硒菌株KX-8为雷氏普罗威登斯菌(Providencia rettgeri);该菌株对亚硒酸钠有较强的耐受能力,最高可达到450 mmol/L;在72 h内,KX-8能把93.16%的亚硒酸钠还原为红色纳米硒。研究还发现KX-8具有较强的IAA分泌能力,分泌量为9.03 mg/L;该菌株也可利用非色氨酸途径产生IAA,在加入500 mg/L的L-色氨酸后IAA的分泌量可达到45.99 mg/L,同时添加不同浓度亚硒酸钠能显著促进KX-8对IAA的分泌。筛选出具有高耐硒能力和强IAA分泌能力的菌株,对于富硒微生物肥料研发、富硒土壤资源开发利用、硒污染土壤环境修复、富硒农产品生产、富硒微生物种质资源的改良具有重要意义。
A strain with high tolerance to sodium selenite was isolated and identified from Se-rich soil of Enshi Tujia and Miao Autonomous Prefecture in Hubei province,its reducing ability to sodium selenite was tested,its biological characteristics of nano-selenium produced by biotransformation were characterized,and its indoleacetic acid(IAA)production capacity was determined.The strain was identified as Providencia rettgeri KX-8 by physiological and biochemical identification and 16S rDNA gene sequencing analysis.The strain had strong tolerance to sodium selenite,up to 450 mmol/L.KX-8 reduced 93.16%sodium selenite to red nano-selenium within 72 h.The study also found that KX-8 has a strong ability to secrete IAA,secreting amount is 9.03 mg/L,the strain can also use non-tryptophan pathway to produce IAA,and the secretion amount of IAA can reach 45.99 mg/L after adding 500 mg/L L-tryptophan.At the same time,different concentrations of sodium selenite can significantly promote the secretion of IAA by KX-8.Screening the strains with high selenium tolerance and strong IAA secretion ability is of great significance for the research and development of selenium-rich microbial fertilizer,the exploitation and utilization of selenium-rich soil resources,the remediation of selenium-polluted soil environment,the production of selenium-rich agricultural products,and the improvement of selenium-rich microbial germplasm resources.
作者
马丽莎
陈小玲
檀艳萍
王璋倩
丛欣
程水源
董星星
何毅
MA Lisha;CHEN Xiaoling;TAN Yanping;WANG Zhangqian;CONG Xin;CHENG Shuiyuan;DONG Xingxing;HE Yi(National R&D Center for Se-Rich Agricultural Products Processing,Hubei Engineering Research Center for Deep Processing of Green Se-Rich Agricultural Products,School of Modern Industry for Selenium Science and Engineering,Wuhan Polytechnic University,Wuhan 430023,China;Enshi Se-Run Material Engineering Technology Co.,Ltd.,Enshi 445000,China)
出处
《食品科技》
CAS
北大核心
2023年第5期1-9,共9页
Food Science and Technology
基金
湖北省自然科学基金项目(2022CFB945)
湖北省教育厅科学技术研究项目(Q20211612)
武汉轻工大学硒科学与工程现代产业学院技术储备项目(Sel-202103)
武汉轻工大学博士科研启动基金项目(2021RZ071)
武汉轻工大学校立科研项目(2022Y41)。
关键词
耐硒
雷氏普罗威登斯菌
纳米硒
吲哚乙酸
selenium tolerance
Providencia rettgeri
selenium nanoparticles
indoleacetic acid
