摘要
目的 探讨采用食蟹猴制备自体腘绳肌腱移植重建前交叉韧带(anterior cruciate ligament,ACL)模型的可行性。方法 取健康成年雄性食蟹猴12只,体质量8~13 kg,随机分为两组(n=6)。其中,实验组动物按照单束腘绳肌腱重建ACL方法制备右下肢膝关节自体腘绳肌腱移植重建ACL模型,对照组仅切断右下肢膝关节ACL。术后观察两组动物存活情况。于术前和术后3、6、12个月,测量两组术侧膝关节活动度、大腿围、小腿围,使用Ligs关节韧带数字体查仪测量13~20 N载荷条件下双膝胫骨前移距离差值(anterior tibial translation Dvalue,ATTD)。同时,实验组行MRI检查,观察重建术后移植物形态,并测量信噪比(signal/noise quotient,SNQ)以评估移植物成熟度。结果 术后两组动物均存活至实验完成;实验组术后膝关节活动度、大腿围、小腿围均呈先降低再逐渐增加趋势,术后3、6个月小于术前(P<0.05),但术后12个月与术前比较差异无统计学意义(P>0.05)。对照组术后膝关节活动度无明显变化,手术前后比较差异均无统计学意义(P>0.05);但术后大腿围、小腿围均逐渐减小(P<0.05),且与术前比较差异有统计学意义(P<0.05)。术后6、12个月实验组大腿围、小腿围均大于对照组(P<0.05),3、6个月膝关节活动度小于对照组(P<0.05)。在13~20 N载荷条件下,实验组术后ATTD均呈先增高再降低趋势,术后3、6个月高于术前(P<0.05),术后12个月与术前比较差异无统计学意义(P>0.05)。而对照组术后3、6、12个月ATTD无明显变化,差异无统计学意义(P>0.05),但均高于术前(P<0.05)。在相同载荷下,术后各时间点实验组ATTD均小于对照组(P<0.05)。实验组MRI检查示,重建后ACL边界逐渐清晰,并被滑膜覆盖;术后各时间点SNQ均高于术前,但随时间延长SNQ逐渐降低,各时间点间差异均有统计学意义(P<0.05)。结论 采用食蟹猴成功建立自体腘绳肌腱移植重建ACL模型。
Objective To investigate the feasibility of establishing an anterior cruciate ligament(ACL)reconstruction model using hamstring tendon autograft in cynomolgus monkeys.Methods Twelve healthy adult male cynomolgus monkeys,weighing 8-13 kg,were randomly divided into two groups(n=6).In the experimental group,the ACL reconstruction model of the right lower limb was prepared by using a single bundle of hamstring tendon,and the ACL of the right lower limb was only cut off in the control group.The survival of animals in the two groups was observed after operation.Before operation and at 3,6,and 12 months after operation,the knee range of motion,thigh circumference,and calf circumference of the two groups were measured;the anterior tibial translation D-value(ATTD)was measured by Ligs joint ligament digital body examination instrument under the loads of 13-20 N,respectively.At the same time,the experimental group underwent MRI examination to observe the graft morphology and the signal/noise quotient(SNQ)was caculated.ResultsAll animals survived to the end of the experiment.In the experimental group,the knee range of motion,thigh circumference,and calf circumference decreased first and then gradually increased after operation;the above indexes were significantly lower at 3 and 6 months after operation than before operation(P<0.05),and no significant difference was found between pre-operation and 12 months after operation(P>0.05).In the control group,there was no significant change in knee range of motion after operation,showing no significant difference between pre-and post-operation(P>0.05),but the thigh circumference and calf circumference gradually significantly decreased with time(P<0.05),and the difference was significant when compared with those before operation(P<0.05).At 6 and 12 months after operation,the thigh circumference and calf circumference were significantly larger in the experimental group than in the control group(P<0.05).At 3 and 6 months after operation,the knee range of motion was significantly smaller in the experimental group than in the control group(P<0.05).Under the loading condition of 13-20 N,the ATTD in the experimental group increased first and then decreased after operation;and the ATTD significantly increased at 3,6 months after operation when compared with the value before operation(P<0.05).But there was no significant difference between the pre-operation and 12 months after operation(P>0.05).There was no significant change in ATTD in the control group at 3,6,and 12 months after operation(P>0.05),and which were significantly higher than those before operation(P<0.05).At each time point after operation,the ATTD was significantly smaller in the experimental group than in the control group under the same load(P<0.05).The MRI examination of the experimental group showed that the ACL boundary gradually became clear after reconstruction and was covered by the synovial membrane.The SNQ at each time point after operation was significantly higher than that before operation,but gradually decreased with time,and the differences between time points were significant(P<0.05).Conclusion TheACL reconstruction model in cynomolgus monkey with autogenous hamstring tendon transplantation was successfully established.
作者
卢晓君
余洋
谢冰
王国梁
杨腾云
熊波涵
刘津瑞
李彦林
LU Xiaojun;YU Yang;XIE Bing;WANG Guoliang;YANG Tengyun;XIONG Bohan;LIU Jinrui;LI Yanlin(Department of Sports Medicine,First Affiliated Hospital of Kunming Medical University,Kunming Yunnan,650032,P.R.China;Department of Obstetrics,First Affiliated Hospital of Kunming Medical University,Kunming Yunnan,650032,P.R.China)
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2023年第7期862-867,共6页
Chinese Journal of Reparative and Reconstructive Surgery
基金
云南省骨关节疾病临床医学中心项目(ZX2019-03-04)
云南省领军人才项目(L-201601)
云南省重大科技专项计划项目(202102AA100015)。
关键词
动物模型
食蟹猴
前交叉韧带重建
自体肌腱移植
腘绳肌腱
Animal model
cynomolgus monkey
anterior cruciate ligament reconstruction
autologous tendon transplantation
hamstring tendon
