摘要
目的:通过射频热凝损伤大鼠坐骨神经,建立热损伤引起的神经病理性疼痛动物模型。方法:取24只健康成年SD大鼠随机分为四组,每组6只。其中假手术组(Sham组)仅进行相关手术操作,RF_(55)组、RF_(65)组和RF_(75)组分别给予坐骨神经55℃、65℃和75℃射频热凝处理,观察术前、术后1~40天机械刺激缩足反射阈值(mechanical withdrawal threshold,MWT)和热缩足反射潜伏期(thermal withdrawal latency,TWL)变化,并判断大鼠自发性痛行为和术后运动功能差异,确定最佳造模温度。另取20只大鼠随机分为假手术组(Sham组)和最佳造模温度65℃射频热凝模型组(RF_(65)组),每组10只。进行模型复制,观察术后14天光镜下坐骨神经形态学变化,RT-PCR法测定大鼠L_(4)~L_(6)背根神经节Nav1.8 mRNA表达。结果:RF_(55)组术后3~10天MWT低于术前;TWL术后7~15天明显缩短。RF_(65)MWT术后5~30天降低;TWL术后7~30天缩短。RF_(75)MWT术后3~15天增高;TWL术后均延长。射频热凝组均在术后出现自发性疼痛行为,运动功能评分降低。坐骨神经65℃射频热凝后,组织水肿明显,空泡增多,Nav1.8 mRNA相对表达量明显高于Sham组。结论:大鼠坐骨神经65℃射频热凝,可以复制稳定的热损伤神经病理性疼痛模型。
Objective:To establish a neuropathic pain model induced by radiofrequency thermal injury of the sciatic nerve in rats.Methods:Twenty-four male adult SD rats were randomly divided into four groups.The animals in sham group received relevant operation,and other three groups were given radiofrequency of the sciatic nerve at 55℃(RF_(55)group),65℃(RF_(65)group)and 75℃(RF_(75)group),respectively.The changes of mechanical withdrawal threshold(MWT)and thermal withdrawal latency(TWL)were observed before operation and within 40 days after radiofrequency,and differences in spontaneous pain behavior and postoperative motor function were evaluated to determine the optimal modeling temperature.Another 20 rats were randomly divided into a sham group and an optimal modeling temperature group(RF_(65)group)for model replication.The morphological changes of the sciatic nerve were observed under light microscopy 14 days after surgery,and the expression of Nav1.8 mRNA in the L_(4)-L_(6)dorsal root ganglia of rats was measured with RT-PCR.Results:The MWT was decreased significantly from 3 to 10 days after operation,and the TWL was significantly shortened from 7 to 15 days after operation in RF_(55).The MWT of the RF_(65)group decreased from 5 to 30 days after operation,and the TWL was shortened from 7 to 30 days after operation.The MWT of the RF_(75)group increased from 3 to 15 days after operation,and the TWL was significantly increased during the observation period.The pathological changes in the successfully established group showed nerve edema,loose structure,and increased vacuoles.The mRNA expression of Nav1.8 in the dorsal root ganglion was significantly higher than that in the sham group.Conclusion:Radiofrequency of sciatic nerve at 65℃can replicate a thermally injured neuropathic pain model in rats.
作者
刘彦朋
张瑞
孙柏
徐赓
黄科昌
任占杰
LIU Yanpeng;ZHANG Rui;SUN Bai;XU Geng;HUANG Kechang;REN Zhanjie(Department of Anesthesiology,Weifang Medical University,Weifang 261053,China;WeiFang Nursing Vocational College,Weifang 261041,China;Department of Pain Management,Affiliated Hospital of Weifang Medical University,Weifang 261000,China)
出处
《中国疼痛医学杂志》
CAS
CSCD
北大核心
2023年第7期495-501,共7页
Chinese Journal of Pain Medicine
