摘要
以分析玉米低聚肽和小麦低聚肽理化性质为基础,对2种低聚肽及玉米低聚肽-Ca螯合物与小麦低聚肽-Fe螯合物2种金属螯合物的抗氧化能力和抗过敏活性进行研究。利用DPPH自由基清除能力、羟自由基清除能力及还原能力测试3种方法评价样品的抗氧化能力,并选择透明质酸酶抑制法评价样品的抗过敏活性。抗氧化能力分析表明,4种样品均有较强的抗氧化能力,且在羟自由基清除能力测试中,2种金属螯合物的羟自由基清除率明显高于对应的低聚肽。抗过敏活性分析表明,在质量浓度<20 mg/mL时,低聚肽金属螯合物的透明质酸酶抑制率远高于对应的低聚肽。这与羟自由基测试的结果类似,可见该条件下样品的抗氧化能力和抗过敏活性存在一定的正相关联系。而质量浓度为20~100 mg/mL时低聚肽的抑制率随浓度明显增大,螯合物的抑制率增长则趋于平缓。螯合物在质量浓度<20 mg/mL条件下同时具有较强的抗氧化能力和抗过敏活性,可在食品药品方向进一步应用发展。
The antioxidant effect and antiallergic activity of corn oligopeptides,wheat oligopeptides,calcium-chelating corn oligopeptides,and ferrum-chelating wheat oligopeptides were studied based on the physicochemical property of two oligopeptides.The ability to scavenge DPPH radical and hydroxyl radical as well as the reductive power and hyaluronidase activity inhibition were analyzed.The antioxidant capacity analysis showed that the four samples had the strong antioxidant capacity,and the hydroxyl radical scavenging ratio of the two chelates was significantly higher than that of the corresponding oligopeptides.The analysis of antiallergic activity indicated that the hyaluronidase inhibition ratio of the chelates was much higher than that of the corresponding oligopeptides when the concentration was less than 20 mg/mL.There was a certain positive correlation between the antioxidant capacity and antiallergic activity of the sample because of the similarity between the results of hydroxyl radical test and the hyaluronidase inhibition test.However,the inhibition ratio of oligopeptides increased significantly at a concentration of 20-100 mg/mL,while the inhibition ratio of chelates tended to be flat.The chelates possess both strong antioxidant capacity and antiallergic activity when the concentration was less than 20 mg/mL,so the chelates can be further applied and developed in the food and medicine area.
作者
卢知浩
赵晓涵
裴晨皓
冯晓文
程青丽
谷瑞增
李国明
刘文颖
LU Zhihao;ZHAO Xiaohan;PEI Chenhao;FENG Xiaowen;CHENG Qingli;GU Ruizeng;LI Guoming;LIU Wenying(China National Research Institute of Food and Fermentation Industries Co.Ltd.,Beijing Engineering Research Center of Protein and Functional Peptides,Beijing 100015,China;School of Biochemical,Beijing City University,Beijing 100083,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2023年第14期35-40,共6页
Food and Fermentation Industries
基金
中国食品发酵工业研究院强院工程培育专项(院强院20-功能肽-505)
国家自然科学基金(31671963)
“十三五”国家重点研发计划(2016YFD0400604)。
关键词
玉米低聚肽
小麦低聚肽
铁
钙
螯合物
抗氧化
抗过敏
corn oligopeptides
wheat oligopeptides
ferrum
calcium
chelate
antioxidant
antiallergic
