摘要
目的:探讨振腹手法对抑郁症大鼠模型骨骼肌组织炎症损伤的干预作用。方法:将32只大鼠随机抽取8只作为正常组,其余大鼠进行3周的慢性不可预见性温和应激方法(CUMS)造模并随机分为模型组、振腹组和氟西汀组,干预4周,在第7周末完成行为学检测,取大鼠骨骼肌组织样本分别检测白细胞介素-1β(IL-1β)、IL-4、IL-6、IL-8、IL-10、肿瘤坏死因子-α(TNF-α)的含量,沉默信息调节因子1(SIRT1)、过氧化物酶体增殖物激活受体γ辅激活物-1α(PCG-1α)、核因子κB(NF-κB)的蛋白及信使核糖核酸(mRNA)含量。结果:第7周末,与模型组大鼠比较,振腹组大鼠的糖水偏好率、绝望时间、总移动距离、穿格次数均显著改善(P<0.01)。第7周末,与模型组大鼠比较,振腹组大鼠的IL-4、IL-10显著升高(P<0.01),IL-1β、IL-6、IL-8、TNF-α含量显著降低(P<0.01);核因子κB蛋白含量下降(P<0.05),mRNA含量显著下降(P<0.01);SIRT1蛋白含量、mRNA含量显著升高(P<0.01);PGC-1α蛋白含量升高(P<0.05),mRNA含量显著升高(P<0.01)。结论:振腹推拿手法可以改善抑郁症模型大鼠的抑郁症状,其作用机制可能是抑制骨骼肌组织核因子κB蛋白的表达,促进SIRT1、PGC-1α蛋白的表达,从而增加抑炎因子的含量,减少炎症介质的含量。
Objective:To investigate the intervention effects of abdominal vibration manipulation on skeletal muscle tissue inflammation in a rat model of depression.Methods:Thirty-two rats were randomly divided into a normal group(n=8)and an experimental group(n=24).The rats in the experimental group underwent chronic unpredictable mild stress(CUMS)for three weeks for model induction.The model rats were further randomly divided into a model group,an abdominal vibration group,and a fluoxetine group.The intervention lasted four weeks,and behavioral tests were conducted at the end of the 7th week.Skeletal muscle tissue samples were collected from the rats to measure the levels of interleukin(IL)-1β,IL-4,IL-6,IL-8,IL-10,tumor necrosis factor(TNF)-α,as well as the protein and messenger RNA(mRNA)levels of silent information regulator 1(SIRT1),peroxisome proliferator-activated receptorγcoactivator 1α(PGC-1α),and nuclear factorκB(NF-κB).Results:At the end of the 7th week,compared with the model group,the abdominal vibration group showed improvements in sucrose preference,despair time,total distance moved,and the number of grid crossings(P<0.01).Additionally,compared with the model group,the abdominal vibration group showed increased levels of IL-4 and IL-10(P<0.01),decreased levels of IL-1β,IL-6,IL-8,and TNF-α(P<0.01),decreased NF-κB protein levels(P<0.05)and mRNA levels(P<0.01),increased SIRT1 protein levels(P<0.01)and mRNA levels(P<0.01),and increased PGC-1αprotein levels(P<0.05)and mRNA levels(P<0.01).Conclusion:Abdominal vibration manipulation can improve depressive symptoms in a rat model of depression.The mechanism of action may involve inhibiting the expression of NF-κB protein in skeletal muscle tissue,promoting the expression of SIRT1 and PGC-1αproteins,thereby increasing the levels of anti-inflammatory factors and reducing the levels of inflammatory mediators.
作者
周可林
董硕
国生
魏培栋
付国兵
杨靖颐
ZHOU Kelin;DONG Shuo;GUO Sheng;WEI Peidong;FU Guobing;YANG Jingyi(Beijing University of Chinese Medicine,Beijing 100029,China;Dongfang Hospital of Beijing University of Chinese Medicine,Beijing 100078,China)
出处
《世界中医药》
CAS
2023年第11期1551-1557,共7页
World Chinese Medicine
基金
国家自然科学基金面上项目(81574092)。
