摘要
目的以当归芍药散入血成分为研究对象,基于网络药理学和动物实验探究当归芍药散治疗原发性痛经的有效性及作用机制。方法运用Gene Cards、OMIM数据库筛选与当归芍药散入血成分对接的原发性痛经靶点,利用R语言获得关键靶标,进行蛋白互作网络构建,针对筛选出的靶点进行GO和KEGG通路富集;利用AutoDock Vina 1.2.3版软件进行分子对接;通过当归芍药散给药干预原发性痛经模型大鼠,从行为学和组织病理学角度验证当归芍药散的有效性。结果共筛选出当归芍药散入血成分12个,作用于肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-6、VEGFA、PTGS2等核心靶点,主要参与IL-17、TNF、花生四烯酸代谢等信号通路。分子对接显示Z-丁烯基酞内酯、洋川芎内酯I、芍药内酯苷3个活性成分与靶标蛋白结合能力较强。动物实验表明,原发性痛经模型大鼠扭体反应明显增加(P<0.05),子宫内膜剥脱及水肿严重;当归芍药散能有效减少大鼠扭体次数(P<0.05),减轻子宫内膜剥脱及水肿。结论当归芍药散主要通过中枢镇痛、调控激素效应、抑制子宫炎症反应等药理作用发挥治疗原发性痛经的效果,为后续研究提供参考。
Objective This study focused on the blood-entering components as the research object,the efficacy and mechanism of Danggui shaoyao powder in treating primary dysmenorrhea were investigated by network pharmacology and animal experiments.Methods Gene Cards and OMIM databases were used to screen the primary dysmenorrhea targets docking with the blood components.The R language was used to obtain key targets,and the protein-protein interaction network was constructed.The GO and KEGG pathways were enriched for the selected targets.Molecular docking was performed using AutoDock Vina 1.2.3 software to verify the effectiveness of Danggui shaoyao powder from the perspective of behavior and histopathology by the intervention of Danggui shaoyao powder in primary dysmenorrhea model rats.Results A total of 12 blood components were screened,which acted on core targets such as TNF,IL6,VEGFA,and PTGS2,and were mainly involved in signaling pathways such as IL-17,TNF,and arachidonic acid metabolism.Molecular docking showed that the three active components,Z-butenylphthalolide,ligustrolide I,and paeonifloridin had strong binding ability to the target protein.Animal experiments showed that the writhing response of primary dysmenorrhea rats was significantly increased(P<0.05),severe endometrial exfoliation and edema were observed;Danggui shaoyao powder can effectively reduce the number of twisting(P<0.05),reduce endometrial exfoliation and edema.Conclusion Danggui shaoyao powder has the effect of treating primary dysmenorrhea mainly through central analgesia,regulating hormone effect,and inhibiting uterine inflammatory reaction,and other pharmacological effects,which provides a reference for subsequent research.
作者
赵蓝青青
李哲
刘孜
李娜
李忠思
刘振华
熊辉
ZHAO Lanqingqing;LI Zhe;LIU Zi;LI Na;LI Zhongsi;LIU Zhenhua;XIONG Hui(Hebei Key Laboratory of Study and Exploitation of Chinese Medicine,Chengde Medical University,Chengde 067000,China;Hebei Key Laboratory of Nerve Injury and Repair,Chengde Medical University,Chengde 067000,China;Institute of Basic Medicine,Chengde Medical University,Chengde 067000,China;Department of Pharmacy,Affiliated Hospital of Chengde Medical University,Chengde 067000,China)
出处
《医药导报》
CAS
北大核心
2023年第8期1110-1116,共7页
Herald of Medicine
基金
国家自然科学基金青年基金项目(82104384)
河北省高等学校科学技术研究项目(QN2021008)
承德医学院高层次人才科研启动基金资助项目(202103)
河北省科技厅“技术创新引导专项-科技工作会商”项目(2021)
承德医学院中药药效物质基础青年PI科技创新团队。
关键词
当归芍药散
原发性痛经
入血成分
网络药理学
有效性
Danggui shaoyao powder
Primary dysmenorrhea
Blood components
Network pharmacology
Validity
