摘要
为挖掘湿地松(Pinus elliottii)松脂合成相关的基因,对不同采脂期的木质部和针叶进行高通量转录组测序,与火炬松(Pinus taeda)参考基因组进行比对,共获得了68211条unigenes,546356450条clean reads,平均比对率达90.21%。将不同时期木质部、木质部与针叶间进行两两对比,以P<0.05,|log2FoldChange|>1.0为标准来筛选差异基因,并进行GO和KEGG富集分析。结果表明,参与萜类物质合成的差异基因有133个,其中大部分富集在MEP途径,从差异基因中挑选8个产脂相关的候选基因进行RT-qPCR验证,确定HMGR、DXS、TPS、ABC转运蛋白基因与产脂存在关联性。通过转录组测序与分析,挖掘出133个参与松脂萜类物质合成相关的差异基因,其中萜烯合酶基因(TPS)和ABC转运基因在正调控萜类物质合成中发挥关键作用。
In order to explore the genes related to pine resin biosynthesis in Pinus elliottii,a total of 12 samples of xylem at different resin collection stages and needles were used for high-throughput transcriptome sequencing.Compared with the reference genome of P.taeda,a total of 68211 unigenes and 546356450 clean reads were obtained with an average mapping rate of 90.21%.The expression profiles between needles and xylem were compared in pairs.The differentially expressed genes(DEGs)were selected according to P<0.05,|log2foldchange|>1.0,and then annotated by GO and KEGG enrichment analysis.The results showed that 133 DEGs involved in terpene synthesis,most of which were enriched in MEP pathway.Eight candidate genes related to pine resin biosynthesis were selected from the DEGs and validated by RT-qPCR,in which HMGR,DXS,TPS,ABC transporter genes were highly correlated with the rosin production.Therefore,133 differential genes related to pine resin biosynthesis were mined through transcriptome sequencing,and among which three TPS genes and two ABC transporter genes positively regulate terpenoid synthesis.
作者
张文娟
谷振军
胡珊
张志红
李火根
杨春霞
ZHANG Wenjuan;GU Zhengjun;HU Shan;ZHANG Zhihong;LI Huogeng;YANG Chunxia(Key Laboratory of Forest Genetics&Biotechnology of Ministry of Education,Co-Innovation Center for Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing 210037,China;Institute of Forest Genetic Breeding and Cultivation,Jiangxi Academy of Forestry,Nanchang 330032,China;Xiajiang County Forest Seed Farm,Ji’an 331409,Jiangxi,China)
出处
《热带亚热带植物学报》
CAS
CSCD
北大核心
2023年第4期531-540,共10页
Journal of Tropical and Subtropical Botany
基金
国家自然科学基金项目(32160389)
江西林业科学院青年科技人才培养项目(2021522901)资助。
关键词
湿地松
松脂
转录组
产脂候选基因
Pinus elliottii
Resin
Transcriptome
Candidate genes involved in resin biosynthesis
