摘要
目的:探讨续断种子方对少精子症模型小鼠附睾结构的影响。方法:将45只中的10只雄性小鼠标记为正常组,余下35只小鼠经腹注射白消安,连续注射5 d后,使用水合氯醛麻醉随机选取的5只小鼠,并取出其左侧附睾,光学显微镜下观察附睾见少量精子,表明成功造模,随机分为模型组、左卡组、续断组3组各组10只。续断组和左卡组依照人体等效剂量溶剂配药灌胃,正常组和模型组予生理盐水灌胃。灌胃8周后处杀全部实验小鼠,并摘取左侧附睾,行精子检测、精浆生化、HE染法色检测,电镜检测附睾。结果:模型组小鼠附睾上皮细胞增大,部分主细胞及基细胞空泡变性,间质细胞水肿,血管扩张。与正常组比较,模型组精液浓度、活力、SOD、α‐葡糖苷酶、果糖显著下降,差异具有统计学意义(P<0.01);药物灌胃治疗8周,续断组相较于模型组在精液浓度、活力、SOD、α‐葡糖苷酶、果糖等方面显著增加,差异具有统计学意义(P<0.01)。结论:续断种子方对于少精子症模型小鼠附睾结构、附睾精子成熟微环境、维护附睾上皮结构稳定性方面有着显著改善,为精子在附睾培育成熟创造条件。
Objective:To investigate the intervention of Xuduan Zhongzi Formula on the epididymis structure of model of oligospermia mice.Methods:Ten of the 45 male mice were labeled as the normal group,and the remaining 35 mice were injected with chloral hydrate for five consecutive days,and the randomly selected five mice were anesthetized with chloral hydrate.The left epididymis was removed,and a few sperm were found in the epididymis under the optical microscope,indicating successful construction of the model.They were randomly divided into three groups:the normal group,the L‐carnitine group and the Xuduan group with 10 rats in each group.The the L‐carnitine group and the Xuduan group were administrated with equivalent dose of human solvent,and the normal group and the model group were administrated with normal saline.After 8 weeks of intragastric administration,all experimental mice were killed,and the left epididymis was extracted for sperm detection,seminal plasma biochemical detection,HE staining,and electron microscopy.Results:In the model group,enlarged epididymal epithelial cells,vacuolar degeneration of primary and basal cells,and edema of interstitial cells and vascular dilation were found.Compared with the normal group,semen concentration,activity,SOD,Α‐glucosidase and fructose in the model group were significantly decreased,and the difference was statistically significant(P<0.01).After eight weeks of drug intragastric treatment,the semen concentra‐tion,activity,SOD,Α‐glucosidase and fructose in the Xuduan group were significantly increased compared with the model group,and the difference was statistically significant(P<0.01).Conclusion:Xuduan Zhongzi Formula can significantly improve the epi‐didymal structure,the microenvironment of epididymal sperm maturation and the stability of epididymal epithelial structure of oli‐gospermia model mice,creating conditions for sperm maturation in the epididymis.
作者
刘金尧
何明
王权胜
唐振宇
杨德芬
LIU Jin‐yao;HE Ming;WANG Quan‐sheng;TANG Zhen‐yu;YANG De‐fen(Guangxi University of Chinese Medicine,Nanning 530200,China;Guilin Hospital of Traditional Chinese Medicine,Guilin 541002,China;The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023,China)
出处
《海南医学院学报》
2023年第14期1069-1074,共6页
Journal of Hainan Medical University
基金
中央引导地方科技发展资金(ZY20198022)
广西中医药大学研究生教育创新计划项目(YCXJ2021067)。
关键词
续断种子方
少精子症
附睾超微结构
精子成熟
Xuduan Zhongzi Formula
Oligospermia
Ultrastructure of epididymis
Sperm maturation
