摘要
【目的】探究土壤中四环素残留对土培黄豆芽的生长发育、根际微生物及营养品质的影响,为正确评估抗生素残留对蔬菜种植业的影响及制定土壤-蔬菜系统中抗生素污染防控策略提供理论基础。【方法】模拟土壤中不同四环素残留水平(0、25、50mg/kg),采用高效液相色谱(high performance liquid chromatography,HPLC)方法测定黄豆芽中的四环素残留量、理化方法测定黄豆芽的营养品质、高通量技术测定根际微生物群落。【结果】黄豆芽中四环素累积量随土壤中四环素残留量的增加而增加,累积量分布表现为根>下胚轴>子叶;四环素残留显著抑制黄豆芽的根和下胚轴的发育及其生物量、维生素C含量和抗氧化性,但增加了纤维素含量。在这些指标中维生素C含量的变化最显著,在黄豆芽生长第5天,25mg/kg和50mg/kg四环素残留组中,黄豆芽中维生素C含量较对照组分别降低41.35%和49.80%;此外,四环素残留显著影响黄豆芽根际微生物群落结构,尤其是与氮循环相关的属。其中,明显增加了不动杆菌属(Acinetobacter)和栖热菌属(Thermus)的相对丰度,减少了假黄色单胞菌属(Pseudoxanthomonas)和氢嗜菌属(Hydrogenophaga)的相对丰度。【结论】土壤中的四环素残留显著影响黄豆芽的生长发育、根际微生物群落结构以及维生素C等重要营养品质指标。
[Objective]To explore the effect of soil tetracycline on the growth and development,rhizosphere microbes,and nutrients of soybean sprouts,and to lay a theoretical basis for evaluating the influence of antibiotic residue on vegetable cultivation and preventing and controlling antibiotic contamination in soil-vegetable system.[Methods]We simulated different levels of tetracycline(0,25,50 mg/kg)in soil and determined the tetracycline in soybean sprouts by high performance liquid chromatography(HPLC).The nutrients in soybean sprouts were measured with physicochemical methods and rhizosphere microbes were elucidated by high-throughput sequencing.[Results]Tetracycline accumulation in soybean sprouts rose with the increase in the level of tetracycline in soil,and the distribution followed the order of root>hypocotyl>cotyledon.Tetracycline residue in soil significantly inhibited the development and biomass of root and hypocotyl,vitamin C synthesis,and antioxidant activity of soybean sprouts,particularly the vitamin C synthesis.To be specific,on the fifth day of growth,vitamin C content in soybean sprouts cultivated in soil with 25 mg/kg and 50 mg/kg tetracycline was 41.35%and 49.80%lower than that of the control,respectively.Moreover,tetracycline residue significantly affected the microbial community structure in rhizosphere of soybean sprouts,especially the nitrogen cycling-related genera.Specifically,the relative abundance of Acinetobacter and Thermus significantly increased and that of Pseudoxanthomonas and Hydrogenophaga reduced.[Conclusion]Tetracycline residue in soil suppressed the growth of soybean sprouts,rhizosphere microbial community structure,and important nutrients such as vitamin C.
作者
蔡婷
张珊
李鑫
向文良
CAI Ting;ZHANG Shan;LI Xin;XIANG Wenliang(Key Laboratory of Food Biotechnology of Sichuan,Institutes of Ancient Fermentation&Brewing Technology,School of Food and Bioengineering,Xihua University,Chengdu 610039,Sichuan,China)
出处
《微生物学报》
CAS
CSCD
北大核心
2023年第7期2864-2879,共16页
Acta Microbiologica Sinica
基金
国家自然科学基金(31571935)
四川省国际合作(澳新)研究院开放课题(AXYJ2022-007)
成都市科技项目(2020-YF09-00007-SN)
西华大学人才引进项目(Z211009)。
关键词
四环素残留
黄豆芽
根际微生物
营养品质
tetracycline residue
soybean sprouts
rhizosphere microbes
nutritional quality
