摘要
目的 探究丙型肝炎患者血清丙型肝炎病毒抗体(HCV-Ab)、微小RNA-27a(miR-27a)和miR-130a表达水平及意义。方法 选取2020年1月至2022年6月就诊于西安市北方医院的152丙型肝炎患者作为观察组,另选取同期在该院体检的健康人员152例作为对照组。测定两组血清miR-27a、miR-130a水平及观察组血清HCV-Ab滴度和HCV-DNA载量。比较两组血清miR-27a、miR-130a水平,分析观察组血清HCV-Ab滴度、miR-27a、miR-130a水平与HCV-DNA载量、肝脏组织炎症活动度和纤维化程度的相关性,探究其在丙型肝炎肝硬化预测中的价值。结果 观察组血清miR-27a、miR-130a水平均显著高于对照组(P<0.01)。观察组血清HCV-Ab滴度、miR-27a、miR-130a水平均与HCV-DNA载量呈正相关性(P<0.01);肝脏炎症G4级患者的血清HCV-Ab滴度、miR-27a、miR-130a水平>G3级>G2级>G1级(P<0.01);肝脏纤维化S4期患者血清HCV-Ab滴度、miR-27a、miR-130a水平>S2~S3期>S1期>S0期(P<0.01)。受试者工作特征曲线分析结果显示,丙型肝炎患者血清HCV-Ab滴度、miR-27a、miR-130a水平均对肝硬化发生具有一定预测价值,且3项指标联合检测的曲线下面积明显高于各指标单独检测(P<0.01)。结论 丙型肝炎患者血清miR-27a、miR-130a表达水平上调,患者HCV-Ab滴度、miR-27a、miR-130a水平均与HCV-DNA载量、肝脏炎症活动度和纤维化严重程度呈一定相关性,故血清miR-27a、miR-130a水平和HCV-Ab滴度对肝硬化具有一定预测价值,且联合检测预测肝硬化效能更高。
Objective To investigate the expression levels and significance of hepatitis C virus antibody(HCV-Ab),microRNA-27A(miR-27a)and microRNA-130a(miR-130a)in serum of patients with hepatitis C.Methods From January 2020 to June 2022,152 cases of hepatitis C(observation group)and 152 healthy physical examination subjects(control group)in Xi′an North Hospital were collected.Serum miR-27a and miR-130a expression levels of the two groups were determined,and serum HCV-Ab titer and HCV-DNA load of the observation group were determined.The serum expression levels of miR-27a and miR-130a in both groups were compared,and the correlation between the serum HCV-Ab titer,miR-27a and miR-130a levels and HCV-DNA load,liver tissue inflammatory activity and fibrosis in the observation group was analyzed,so as to explore their value in the prediction of hepatitis C cirrhosis.Results The levels of miR-27a and miR-130a in the observation group were significantly higher than those in the control group(P<0.01).The levels of serum HCV-Ab titer,miR-27a and miR-130a were positively correlated with HCV-DNA load in the observation group(P<0.01);the serum HCV-Ab titer,miR-27a and miR-130a levels of patients with liver inflammation in G4>G3>G2>G1(P<0.01);the serum HCV-Ab titer,miR-27a and miR-130a levels of the patients with fibrosis in S4 stage>S2-S3 stage>S1 stage>S0 stage(P<0.01).Receiver operating characteristiccurve analysis showed that serum HCV-Ab titer,miR-27a and miR-130a levels of hepatitis C patients had certain predictive value for the occurrence of cirrhosis,and the aera under the curve for combined detection of the three indexes was significantly higher than that for single detection of each index(P<0.01).Conclusion The expression lelves of serum miR-27a and miR-130a in serum of hepatitis C patients are up-regulated,and the expression levels of HCV-Ab titer,miR-27a and miR-130a are all correlated with HCV-DNA load,liver inflammatory activity and fibrosis severity.Serum miR-27a,miR-130a and HCV-Ab titer have certain predictive value for cirrhosis,and the combined detection has higher predictive value for cirrhosis.
作者
宋娟芳
李源
SONG Juanfang;LI Yuan(Department of Clinical Laboratory,Shaanxi Xi′an North Hospital,Xi′an,Shaanxi 710043,China)
出处
《检验医学与临床》
CAS
2023年第15期2238-2242,共5页
Laboratory Medicine and Clinic
