摘要
为了探究乌苏里貉黑素皮质素受体3(melanocortin 3 receptor,MC3R)的蛋白结构和生理功能,试验采用生物信息学方法对乌苏里貉MC3R蛋白的理化性质、信号肽和跨膜结构、磷酸化修饰位点、二级结构、保守结构域、三级结构、亚细胞定位、蛋白互作网络、功能注释进行分析,并通过构建基于MC3R蛋白氨基酸序列的系统发育树对乌苏里貉与其他物种的亲缘关系进行分析。结果表明:乌苏里貉MC3R蛋白由323个氨基酸组成,分子式为C_(1640)H_(2562)N_(400)O_(428)S_(33),相对分子质量为35788.79,是一种稳定的酸性疏水性蛋白。该蛋白不具有信号肽,为一种非分泌的跨膜蛋白,具有7个跨膜螺旋区域,含有15个潜在磷酸化位点;二级结构由54.49%的α-螺旋、25.70%的无规则卷曲、16.41%的延伸链和3.41%的β-转角构成,包含一段G蛋白偶联受体超家族所共有的保守结构;三级结构以人MC4R蛋白为模板进行同源建模,经检测建模质量较高;该蛋白主要分布在细胞膜中,然后是内质网,再次是液泡,而在高尔基体、细胞核、细胞骨架中分布较少;与阿片黑素促皮质激素原(proopiom elanocortin,POMC)、MC4R、MC2R、Agouti相关蛋白(Agouti related protein,AgRP)等11种蛋白存在互作关系。在分子功能方面,该蛋白具有促黑激素受体活性,能够与神经肽结合;在生物学过程中,该蛋白参与G蛋白偶联受体介导的信号通路,以及基因表达的生理调控、运动调节和摄食行为调节等过程;在细胞组分方面,该蛋白主要分布在细胞膜中。乌苏里貉与犬和赤狐的亲缘关系较近,与山羊、绵羊、羚羊、驴、马鹿和水牛的亲缘关系相对较远。说明乌苏里貉MC3R蛋白可能作为一种信号传导膜受体,参与机体的摄食行为调控等过程。
In order to investigate the protein structure and physiological function of melanocortin 3 receptor(MC3R)in Ussuri raccoon dogs,the physicochemical properties,signal peptide and transmembrane structure,phosphorylation modification site,secondary structure,conserved domain,tertiary structure,subcellular localization,protein interaction network and functional annotation of MC3R protein in Ussuri raccoon dogs were analyzed by bioinformatics method.Phylogenetic tree was constructed based on MC3R protein amino acid sequence to predict the relationship between raccoon and other species.The results showed that MC3R protein in Ussuri raccoon dogs was composed of 323 amino acids;the molecular formula was C1640 H2562 N400428 S33,and the relative molecular weight was 35788.79.It was a stable acid hydrophobic protein.This protein had no signal peptide and was a non-secreted transmembrane protein.It had 7 transmembrane helical regions and 15 potential phosphorylation sites.The secondary structure consisted of 54.49%α-helices,25.70%random curls,16.41%extended chains and 3.41%β-turns,and also contained a conserved structure common to the G-protein-coupled receptor superfamily.Human MC4R protein was used as the template for homologous modeling of tertiary structure,and the quality of modeling was higher.The protein was mainly distributed in the cell membrane,then in the endoplasmic reticulum,and again in the vacuole,but was less distributed in the Golgi apparatus,nucleus,and cytoskeleton.The protein interacted with 11 kinds of proteins,including proopiom elanocortin(POMC),MC4R,MC2R and Agouti related protein(AgPR).In terms of molecular function,this protein had the activity of promoting melanin receptor and could bind to neuropeptides.In biological processes,this protein was involved in G-protein-coupled receptor-mediated signaling pathway,physiological regulation of gene expression,motor regulation and feeding behavior regulation.In terms of cell components,MC3R protein was mainly distributed in cell membranes.Ussuri raccoon dogs were more closely related to Canis lupus familiaris and Vulpes vulpes,but were relatively distantly related to Capra hircus,Ovis aries,Tragelaphus oryx,Kobus leche,Cervus canadensis and Bubalus bubalis.These results suggested that MC3R protein of Raccoon dogs might be involved in the regulation of feeding behavior as a signal transduction membrane receptor.
作者
李鑫
李伟
刘洁
刘进军
王朋然
韩学良
任二军
LI Xin;LI Wei;LIU Jie;LIU Jinjun;WANG Pengran;HAN Xueliang;REN Erjun(Shijiazhuang Academy of Agricultural and Forestry Sciences,Shijiazhang 050041,China;Technological Innovation Center for Fur Animal Breeding of Hebei,Shijiazhuang 050041,China)
出处
《黑龙江畜牧兽医》
北大核心
2023年第14期119-124,131,140,141,共9页
Heilongjiang Animal Science And veterinary Medicine
基金
河北省重点研发计划项目(21326357D)
石家庄市财政预算专项。
关键词
乌苏里貉
MC3R蛋白
生物信息学
蛋白结构
生理功能
Ussuri raccoon dog
MC3R protein
bioinformatics analysis
protein structure
physiological function
