摘要
为构建烟草全基因组模块库,开展烟草分子模块育种,以K326为轮回亲本,烤烟OX2028和香料烟Samsun为供体亲本,经过杂交、连续多代回交、自交,最终获得两套以K326为背景,覆盖供体亲本整个基因组的分子模块库。分子模块的基因组大部分回复到了轮回亲本K326,其田间农艺及抗病性等重要性状偏向亲本K326,不同材料间存在较广泛的遗传变异;群体的基本农艺性状呈现连续的正态或近似正态分布,符合数量性状的表型分布特性;鉴定了7个重要性状显著改变的分子模块,其中较K326中上部叶片开片明显改善的材料32份,叶数明显提高的材料25份,TMV、CMV、PVY病毒病抗性明显提高的材料分别为111、25和5份,黑胫病、青枯病抗性显著提高的材料44和52份。构建的烟草分子模块库可用于后续开展烟草重要性状的QTL定位和基因功能研究。
To construct a tobacco whole-genome module library and carry out tobacco molecular module breeding,in this study we used K326 as the recurrent parent and flue-cured tobacco OX2028 and aromatic tobacco Samsun as donor parents.After hybridization,continuous multi-generation backcrossing,and selfing,two sets of molecular module libraries covering the entire genome of the donor parents were obtained with K326 as the background.The genome of the molecular module mostly returned to the recurrent parent K326,and field agronomic and disease resistance traits were biased towards the parent K326.However,there was still a wide range of genetic variation.Among them,the coefficient of variation for waist leaf length was the smallest for two consecutive years,and the coefficient of variation for internode length was the largest for two consecutive years.The basic agronomic traits of the population showed a continuous normal or near-normal distribution,which is consistent with the phenotypic distribution characteristics of quantitative traits.Seven molecular modules with significant changes in important traits were identified.Compared to K326,32 materials with significantly improved upper leaf opening,25 materials with significantly increased leaf number,111,25,and 5 materials with significantly increased resistance to TMV,CMV,and PVY virus diseases,and 44 and 52 materials with significantly increased resistance to black shank and bacterial wilt diseases were identified.The tobacco molecular module library constructed in this study can be used for subsequent QTL mapping and gene function research on important tobacco traits.
作者
王永达
宋时洋
蒋彩虹
潘旭浩
耿锐梅
任民
刘旦
蒲文宣
刘万峰
王东
毛辉
余祥文
昝艳君
程立锐
杨爱国
WANG Yongda;SONG Shiyang;JIANG Caihong;PAN Xuhao;GENG Ruimei;REN Min;LIU Dan;PU Wenxuan;LIU Wanfeng;WANG Dong;MAO Hui;YU Xiangwen;ZAN Yanjun;CHENG Lirui;YANG Aiguo(Tobacco Research Institute,Chinese Academy of Agricultural Sciences,Qingdao 266101,China;China Tobacco Hunan Industry Company,Changsha 410014,China;Xiangxi Autonimous Prefecture Tobacco Company of Hunan Province,Longshan County Autonimous Prefecture,Longshan,Hunan 416800,China;Sichuan Branch of China National Tobacco Corporation,Chengdu 610000,China)
出处
《中国烟草科学》
CSCD
北大核心
2023年第3期1-9,共9页
Chinese Tobacco Science
基金
中国烟草总公司烟草基因组计划和生物育种重大科技项目[110202101001(JY-01)、110202001024(JY-07)、110202001027(JY-10)]
中国农业科学院科技创新工程(ASTRP-TRI01)。
关键词
烟草
分子模块
设计育种
数量性状
tobacco
molecular module
design breeding
quantitative traits
